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首页> 外文期刊>The American Journal of Tropical Medicine and Hygiene >Plasmodium vivax Sporozoite Production in Anopheles albimanus Mosquitoes for Vaccine Clinical Trials
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Plasmodium vivax Sporozoite Production in Anopheles albimanus Mosquitoes for Vaccine Clinical Trials

机译：疟原虫间日疟原虫子孢子生产的疫苗临床试验。

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摘要

Vaccine development for Plasmodium vivax malaria is underway. A model to assess the protective efficacy of vaccine candidates in humans is urgently needed. Given the lack of continuous P. vivax cultures, we developed a system to infect Anopheles albimanus mosquitoes using blood from P. vivax-infected patients and determined parameters for challenge of malaria-naive volunteers by mosquito bite. Absence of co-infections in parasitized blood was confirmed by tests consistent with blood bank screening. A total of 119 experiments were conducted using batches of 900-4,500 mosquitoes fed by an artificial membrane feeding method. Optimal conditions for mosquito probing and infection were determined. Presence of oocyst and sporozoites were assessed on Days 7-8 and 14-15, respectively, and conditions to choose batches of infected mosquitoes for sporozoite challenge were established. Procedures to infect volunteers took a 2-hour period including verification of inoculum dose. Anopheles albimanus mosquitoes represent a valuable resource for P. vivax sporozoite challenge of volunteers.
机译：间日疟原虫疟疾的疫苗开发正在进行中。迫切需要一种评估候选疫苗对人的保护功效的模型。鉴于缺乏间日疟原虫连续培养，我们开发了一种使用间日疟原虫感染患者的血液感染白带按蚊的系统，并确定了由蚊虫叮咬对未接受疟疾的志愿者发起攻击的参数。通过与血库筛查相一致的试验证实了寄生虫血液中没有合并感染。使用通过人工膜喂食方法喂食的900-4,500批蚊子共进行了119个实验。确定了蚊子探测和感染的最佳条件。分别在第7-8天和第14-15天评估卵囊和子孢子的存在，并建立条件以选择一批受感染的蚊子来挑战子孢子。感染志愿者的程序花了2个小时，包括验证接种剂量。 Albimanus蚊子代表了间日疟原虫子孢子激发志愿者的宝贵资源。

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