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DEVELOPMENT OF A STAND-ALONE AFFINITY CLEAN-UP FOR LYSERGIC ACID DIETHYLAMIDE IN URINE

机译:尿中麦角酸二乙酰胺单链亲和性纯化的研究

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摘要

A total analysis scheme for lysergic acid diethylamide (LSD) from human urine is described. A simple ELISA technique led to the development and optimization of an affinity clean-up cartridge, resulting in high purification factors with a single combined extraction/clean-up step. LSD can be measured with a straightforward HPI,C-fluorescence technique, which minimizes operating complexity and process implementation time. The method has been applied to urine containing 0.5 ng ml(-1) LSD, and the ability of high-affinity materials to preconcentrate a sample into a small volume should allow the working range of the procedure to be adjusted as required. [References: 25]
机译:描述了人尿中麦角酸二乙酰胺(LSD)的总分析方案。一种简单的ELISA技术导致亲和性纯化柱的开发和优化,并通过一个单独的提取/纯化步骤实现了高纯化因子。 LSD可以通过简单的HPI,C荧光技术进行测量,从而最大程度地降低了操作复杂度和过程实施时间。该方法已应用于包含0.5 ng ml(-1)LSD的尿液,并且高亲和力材料将样品预浓缩为小体积的能力应允许根据需要调整操作的工作范围。 [参考:25]

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