...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Efficient enrichment of glycopeptides using metal– organic frameworks by hydrophilic interaction chromatography
【24h】

Efficient enrichment of glycopeptides using metal– organic frameworks by hydrophilic interaction chromatography

机译：利用金属-有机骨架通过亲水相互作用色谱法有效富集糖肽

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Selective enrichment of glycopeptides from complicated biological samples is critical for glycoproteomics to obtain the structure and glycosylation information of glycoproteins using mass spectrometry (MS), which still remains a great challenge. Hydrophilic interaction chromatography (HILIC)-based strategies have been proposed for selective isolation of glycopeptides via the interactions between the glycan of glycopeptides and the matrices. However, the application of these methods is limited by the medium selectivity of HILIC matrices. In this study, hydrophilic metal–organic frameworks (MOFs) were fabricated and used as a HILIC matrix. The cross-linked CD-MOFs (LCD-MOFs) were facilely prepared with γ-cyclodextrin as ligand and possessed nano-sized cubic structure, superior hydrophilicity, and bio-compatibility. The LCD-MOFs performance for the selective enrichment of glycopeptides from the complex biological samples were investigated with a digested mixture of human immunoglobulin G (IgG) that was used as standard samples. In the selectivity assessment, the non-glycopeptides causing ion suppression to the glycopeptides were effectively removed, the signal of glycopeptides were enhanced significantly by LCD-MOFs, and twenty glycopeptides were identified with 67 fmol of IgG digest. In addition, the resulting LCD-MOFs demonstrated the lower detection limit (3.3 fmol) with a satisfactory recovery yield (84–103%) for glycopeptide enrichment from a digest of IgG. Furthermore, a promising protocol was developed for the selective enrichment of glycopeptides from mouse liver, and 344 unique N-glycosylation sites that mapped to 290 different glycoproteins were identified in a single MS run. The results clearly demonstrated that when used in a HILIC matrix, LCD-MOFs have great potential for identifying and enriching low-abundant glycopeptides in complex biological samples.
机译：从复杂的生物样品中选择性富集糖肽对于糖蛋白组学使用质谱（MS）获得糖蛋白的结构和糖基化信息至关重要，这仍然是一个巨大的挑战。已经提出了基于亲水相互作用色谱（HILIC）的策略，用于通过糖肽聚糖与基质之间的相互作用选择性分离糖肽。但是，这些方法的应用受到HILIC矩阵的中等选择性的限制。在这项研究中，亲水金属有机骨架（MOFs）被制成并用作HILIC基质。以γ-环糊精为配体容易地制备了交联的CD-MOFs（LCD-MOFs），并具有纳米级的立方结构，优异的亲水性和生物相容性。用人免疫球蛋白G（IgG）的消化混合物作为标准样品，研究了从复杂生物样品中选择性富集糖肽的LCD-MOFs性能。在选择性评估中，有效去除了对糖肽造成离子抑制的非糖肽，LCD-MOF显着增强了糖肽的信号，并用67 fmol的IgG消化物鉴定出20个糖肽。此外，所得的LCD-MOFs显示出较低的检测限（3.3 fmol），令人满意的回收率（84–103％）来自IgG消化液中的糖肽富集。此外，已开发出一种有前途的方案，用于从小鼠肝脏中选择性富集糖肽，并且在一次MS运行中鉴定出344个映射到290种不同糖蛋白的N-糖基化位点。结果清楚地表明，当在HILIC基质中使用时，LCD-MOF在鉴定和富集复杂生物样品中的低丰度糖肽方面具有巨大的潜力。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号