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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Quantitative real-time detection of carcinoembryonic antigen (CEA) from pancreatic cyst fluid using 3-D surface molecular imprinting
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Quantitative real-time detection of carcinoembryonic antigen (CEA) from pancreatic cyst fluid using 3-D surface molecular imprinting

机译：使用3-D表面分子印迹技术实时定量检测胰腺囊肿液中的癌胚抗原（CEA）

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摘要

In this study, a sensitive, yet robust, biosensing system with real-time electrochemical readout was developed. The biosensor system was applied to the detection of carcinoembryonic antigen (CEA), which is a common marker for many cancers such as pancreatic, breast, and colon cancer. Real time detection of CEA during a medical procedure can be used to make critical decisions regarding further surgical intervention. CEA was templated on gold surface (RMS roughness similar to 3-4 nm) coated with a hydrophilic self-assembled monolayer (SAM) on the working electrode of an open circuit potentiometric network. The subsequent removal of template CEA makes the biosensor capable of CEA detection based on its specific structure and conformation. The molecular imprinting (MI) biosensor was further calibrated using the potentiometric responses in solutions with known CEA concentrations and a detection limit of 0.5 ng ml(-1) was achieved. Potentiometric sensing was then applied to pancreatic cyst fluid samples obtained from 18 patients when the cyst fluid was also evaluated using ELISA in a certified pathology laboratory. Excellent agreement was obtained between the quantitation of CEA obtained by both the ELISA and MI biosensor detection for CEA. A 3-D MI model, using the natural rms roughness of PVD gold layers, is presented to explain the high degree of sensitivity and linearity observed in those experiments.
机译：在这项研究中，开发了具有实时电化学读数的灵敏但坚固的生物传感系统。该生物传感器系统被用于检测癌胚抗原（CEA），它是许多癌症（例如胰腺癌，乳腺癌和结肠癌）的常见标记。在医疗过程中对CEA的实时检测可用于做出有关进一步手术干预的关键决策。将CEA模板化在金表面（RMS粗糙度类似于3-4 nm）上，该表面涂有亲水性自组装单层（SAM），位于开路电位网络的工作电极上。随后移除模板CEA，使生物传感器能够基于其特定结构和构象进行CEA检测。使用电位响应在已知CEA浓度的溶液中进一步校准分子印迹（MI）生物传感器，并实现了0.5 ng ml（-1）的检测限。然后，当在认证的病理实验室中也使用ELISA对囊液进行评估时，对从18位患者获得的胰腺囊液样品应用电位传感。 ELISA和MI生物传感器检测CEA所得的CEA定量结果之间获得了极好的一致性。提出了使用PVD金层的自然均方根粗糙度的3-D MI模型，以解释在这些实验中观察到的高度灵敏度和线性。

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