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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Comparison of FTIR transmission and transfection substrates for canine liver cancer detection
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Comparison of FTIR transmission and transfection substrates for canine liver cancer detection

机译：FTIR传输和转染底物在犬肝癌检测中的比较

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FTIR spectroscopy is a widely used technique that provides insights into disease processes at the molecular level. Due to its numerous advantages it is becoming an increasingly powerful tool for the study of biological materials and has the potential to become an excellent diagnostic method, especially considering the low cost of transflection substrates. However, questions about the usefulness of the transflection measurement mode due to the complicated nature of physical processes occurring during the measurement and in particular the Electric Field Standing Wave (EFSW) effect have been raised. In this paper we present a comparison of the two most common FT-IR measurement modes: transmission and transfection using healthy and pathologically altered tissue (histiocytic sarcoma). We found that the major differences between normal and cancerous tissue were associated with changes DNA and carbohydrate content. In particular we identified a band at 964 cm(-1) assigned to a nucleic acid phosphodiester backbone mode, which appeared more pronounced in cancerous tissue irrespective of the substrate. We applied Principal Component Analysis, Unsupervised Hierarchical Cluster Analysis and k-means clustering to transmission and transflection substrates and found that both measurement modes were equally capable of discrimination normal form cancerous tissue. Moreover, the differences between spectra from cancerous and normal tissue were significantly more important than the ones arising from the measurement modes.
机译：FTIR光谱学是一种广泛使用的技术，可在分子水平上洞悉疾病过程。由于其众多的优势，它正成为研究生物材料的一种日益强大的工具，并且有可能成为一种出色的诊断方法，尤其是考虑到透射反射基板的低成本。然而，由于在测量期间发生的物理过程的复杂性质，尤其是电场驻波（EFSW）效应，引起了关于透射测量模式的有用性的问题。在本文中，我们对两种最常见的FT-IR测量模式进行了比较：使用健康且经过病理改变的组织（组织细胞肉瘤）进行传播和转染。我们发现正常组织和癌组织之间的主要差异与DNA和碳水化合物含量的变化有关。特别是，我们确定了在964 cm（-1）处的一条带分配给核酸磷酸二酯主链模式，该条带在癌性组织中表现得更加明显，与底物无关。我们将主成分分析，无监督层次聚类分析和k-均值聚类应用于透射和透射反射底物，发现这两种测量模式均具有区分正常形态癌组织的能力。此外，来自癌组织和正常组织的光谱之间的差异比由测量模式引起的光谱差异更为重要。

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