...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Dispersive liquid-liquid microextraction using functionalized Mg(OH) _2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS
【24h】

Dispersive liquid-liquid microextraction using functionalized Mg(OH) _2 NPs with oleic acid as hydrophobic affinity probes for the analysis of hydrophobic proteins in bacteria by MALDI MS

机译：使用功能化的Mg（OH）_2 NP与油酸作为疏水亲和探针进行分散液-液微萃取，用于通过MALDI MS分析细菌中的疏水蛋白

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report a dispersive liquid-liquid microextraction (LLME) method using surface modified Mg(OH) _2 nanoparticles (NPs) with oleic acid (OA) as hydrophobic affinity probes for the extraction and preconcentration of hydrophobic biomolecules prior to MALDI MS analysis. OA-capped Mg(OH) _2 NPs were characterized by FT-IR, SEM and TEM. The functionalized Mg(OH) _2 NPs are well dispersed in toluene with an average diameter of <35 nm. Under the optimum conditions, the method showed good linearity in the ranges of 10-400 nM and 0. 1-2.0 μM for valinomycin and gramicidin D, respectively. The limits of detection were in the region of 2.0 nM and 24 nM for valinomycin and gramicidin D, respectively. Moreover, the OA-capped Mg(OH) _2 NPs-assisted LLME method was successfully used for the rapid extraction/preconcentration of hydrophobic proteins in bacteria (Escherichia coli and Bacillus subtilis) prior to their identification by MALDI MS.
机译：我们报告了一种分散的液-液微萃取（LLME）方法，该方法使用表面改性的Mg（OH）_2纳米粒子（NPs）与油酸（OA）作为疏水亲和探针，用于在MALDI MS分析之前提取和预浓缩疏水生物分子。通过FT-IR，SEM和TEM表征了OA封端的Mg（OH）_2 NPs。功能化的Mg（OH）_2 NPs分散在甲苯中，平均直径<35 nm。在最佳条件下，该方法在缬氨霉素和短杆菌肽D的10-400 nM和0. 1-2.0μM范围内显示出良好的线性。瓦里霉素和短杆菌肽D的检出限分别为2.0 nM和24 nM。此外，OA覆盖的Mg（OH）_2 NPs辅助LLME方法已成功用于细菌（大肠杆菌和枯草芽孢杆菌）中疏水蛋白的快速提取/预富集，然后通过MALDI MS对其进行鉴定。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号