Dot-blot amperometric genosensor for detecting a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus

M.I.Pividori; A.Merkoci; S.Alegret

首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >Dot-blot amperometric genosensor for detecting a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus

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Dot-blot amperometric genosensor for detecting a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus

机译：用于检测金黄色葡萄球菌＃beta＃-内酰胺酶耐药性的新决定因素的斑点印迹电流传感器

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A new electrochemical hybridisation genosensor for the detection of resistant bacteria has been developed. This device relies on the immobilisation of a 50-mer oligonucleotide target, unique to a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus, onto an electrochemical transducer. This genosensor is based on a concept adapted from classical dot-blot DNA analysis, but implemented in an electrochemical biosensor configuration. Amperometric transduction and a enzyme label method, that increases the genosensor sensitivity, are the main features of this new approach. In addition to the adapted dot-blot format, a double hybridisation assay, in which two different labelled probes were used, is reported. This procedure, if combined with polymerase chain reaction (PCR), allows determination of the genotype of an antibiotic-resistant organism in a shorter time than that required to perform traditional phenotypic susceptibility testing. Its characteristic are ideal for implementation in a kit form.

机译：已经开发出一种用于检测抗性细菌的新型电化学杂交基因传感器。该设备依赖于将一个50-mer寡核苷酸靶标固定在电化学传感器上，该靶标对金黄色葡萄球菌中＃β＃-内酰胺酶耐药性的新决定因子具有独特性。该基因传感器基于从经典斑点印迹DNA分析改编而成的概念，但以电化学生物传感器配置实现。安培转导和酶标记法增加了基因传感器的灵敏度，是这种新方法的主要特征。除了适应的斑点印迹形式，还报道了使用两种不同标记探针的双重杂交测定法。如果将该程序与聚合酶链反应（PCR）结合使用，则可以比进行传统的表型敏感性测试所需的时间更短，从而可以确定抗生素抗性生物的基因型。它的特性非常适合以套件形式实施。

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《The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry》 |2001年第9期|共7页
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M.I.Pividori; A.Merkoci; S.Alegret;
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1. Dot-blot amperometric genosensor for detecting a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus [J] . M.I.Pividori, A.Merkoci, S.Alegret The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry . 2001,第9期

机译：用于检测金黄色葡萄球菌＃beta＃-内酰胺酶耐药性的新决定因素的斑点印迹电流传感器
2. Spontaneous deletion of the methicillin resistance determinant, mecA, partially compensates for the fitness cost associated with high-level vancomycin resistance in Staphylococcus aureus. [J] . Noto MJ, Fox PM, Archer GL Antimicrobial agents and chemotherapy. . 2008,第4期

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3. Performance of CHROMagar Selective Medium and Oxacillin Resistance Screening Agar Base for Identifying Staphylococcus aureus and Detecting Methicillin Resistance [J] . Jan Kluytmans, Arjanne Van Griethuysen, Piet Willemse, Journal of Clinical Microbiology . 2002,第7期

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4. Synthesis and Characterization of Biogenic Selenium Nanoparticles with Antimicrobial Properties Made by Staphylococcus aureus, Methicillin-resistant Staphylococcus aureus (MRSA), Escherichia coli and Pseudomonas aeruginosa. [C] . David Medina, Thomas J. Webster Society for Biomaterials annual meeting and exposition . 2018

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Dot-blot amperometric genosensor for detecting a novel determinant of #beta#-lactamase resistance in Staphylococcus aureus

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