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首页> 外文期刊>The Analyst: The Analytical Journal of the Royal Society of Chemistry: A Monthly International Publication Dealing with All Branches of Analytical Chemistry >The impact of column inner diameter on chromatographic performance in temperature gradient liquid chromatography
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The impact of column inner diameter on chromatographic performance in temperature gradient liquid chromatography

机译：温度梯度液相色谱柱内径对色谱性能的影响

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摘要

The impact of column inner diameter on chromatographic in temperature gradient liquid chromatography has been investigated in the present study.Columns with inner diameters of 0.32,0.53,3.2 and 4.6 mm were compared with respect to retention and efficiency characteristics using temperature gradients from 30 to 90deg C with temperature ramps of 1,5,10 and 20deg C min~(-1).The columns were all of 15 cm length and were packed with 3 mum Hypersil ODS particles.Alkylbenzenes served as model compounds,and the mobile phase consisited of acetonitrile-water (50:50,v/v).The study revealed that the column ID is not a critical limiting factor when performing temperature programming in LC,at for columns narrower than 4.6 mm inner diameter in the temperature interval 30-90deg C.The retention times for all components on all columns were highly comparable,with similar peak profiles without any signs of peak splitting.The use of mobile phase pre-heating when using the larger bore columns was avoided by starting the temperature gradients close to ambient.However,the relative apparent efficiency was inversely proportional to column inner diameter,making the capillary columns generally more functional towards temperature gradients than the larger bore columns with respect to chromatographic efficiency.In addition,the capillary columns possessed higher robustness towards temperature programming than the conventional columns.
机译：本研究研究了色谱柱内径对温度梯度液相色谱中色谱的影响。比较了使用30至90度温度梯度下内径为0.32、0.53、3.2和4.6 mm的色谱柱的保留和效率特性C，温度梯度为1,5,10和20°C min〜（-1）。柱长均为15 cm，装有3 mum Hypersil ODS颗粒。烷基苯为模型化合物，流动相为乙腈-水（50：50，v / v）研究表明，在30-90°C的温度范围内，对于内径小于4.6 mm的色谱柱，在LC中进行温度编程时，色谱柱ID不是关键的限制因素。所有组分在所有色谱柱上的保留时间具有可比性，峰形相似，没有任何峰分离迹象。使用大口径色谱柱可避免使用流动相预热，因为相对表观效率与色谱柱内径成反比，因此相对于色谱效率，毛细管柱通常比大口径色谱柱对温度梯度的功能更大。此外，毛细管柱还具有与常规色谱柱相比，对温度编程的稳定性更高。

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