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Biomimetic glucose recognition using molecularly imprinted polymer hydrogels

机译：使用分子印迹聚合物水凝胶进行仿生葡萄糖识别

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摘要

Non-covalent molecular imprinting of poly(allylamine hydrochloride) (PAA - HCl) with D-glucose 6-phosphate monobarium salt (GPS-Ba) produced molecularly imprinted polymer hydrogels (MIP) having an affinity to glucose over fructose. The hydrogels were formed by ionic association of the template molecule, GPS-Ba, to the polymer, prior to covalent crosslinking using epichlorohydrin (EPI). The template was removed by an aqueous base wash. Batch equilibration studies using different MIP hydrogels and non-molecularly imprinted polymers (NIPs) were performed in aqueous and buffered media to determine the binding capacities and isomeric selectivities with respect to the sugars, glucose and fructose. MIP glucose hydrogels exhibited binding capacities in excess of 0.6 g of glucose per g of dry gel in a 100% D1 H2O glucose solution, and in a 50-50% glucose-fructose solution mixture. Equilibrium binding capacities of fructose were lower than those observed with respect to glucose, indicating an isomeric preference for the binding of glucose over fructose. These hydrogels demonstrated a remarkable degree of biomimetic sugar recognition to specifically and selectively bind glucose in their swollen state in environments mimicking physiological conditions. (C) 2003 Elsevier Ltd. All rights reserved. [References: 13]
机译：聚（烯丙胺盐酸盐）（PAA-HCl）与D-葡萄糖6-磷酸一钡盐（GPS-Ba）的非共价分子印迹产生了与果糖相比对葡萄糖具有亲和力的分子印迹聚合物水凝胶（MIP）。在使用表氯醇（EPI）共价交联之前，通过模板分子GPS-Ba与聚合物的离子缔合形成水凝胶。通过含水碱洗除去模板。在水和缓冲介质中使用不同的MIP水凝胶和非分子印迹聚合物（NIP）进行批次平衡研究，以确定相对于糖，葡萄糖和果糖的结合能力和异构选择性。在100％D1 H2O葡萄糖溶液和50-50％葡萄糖-果糖溶液混合物中，MIP葡萄糖水凝胶的结合能力超过每g干凝胶0.6 g葡萄糖。果糖的平衡结合能力低于观察到的葡萄糖结合能力，这表明与果糖相比，葡萄糖的结合异构体偏爱。这些水凝胶在模仿生理条件的环境中表现出了显着程度的仿生糖识别能力，能够以溶胀状态特异性和选择性地结合葡萄糖。 （C）2003 Elsevier Ltd.保留所有权利。 [参考：13]

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