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首页> 外文期刊>The European Journal of Neuroscience >Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis.
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Involvement of Ngn2, Tbr and NeuroD proteins during postnatal olfactory bulb neurogenesis.

机译：Ngn2，Tbr和NeuroD蛋白在产后嗅球神经发生过程中的参与。

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摘要

Postnatal neurogenesis takes place in two brain regions, the hippocampus and the subventricular zone (SVZ). The transcriptional cascade controlling hippocampal neurogenesis has been described in detail; however, the transcriptional control of olfactory bulb neurogenesis is still not well mapped. In this study, we provide insights into the molecular events controlling postnatal olfactory bulb neurogenesis. We first show the existence of diverse neural stem cell/progenitor populations along the SVZ-rostral migratory stream (RMS) axis, focusing on those expressing the basic helix-loop-helix (bHLH) transcription factor Mash1. We provide evidence that Mash1-derived progenies generate oligodendrocytic and neuronal precursors through the transient expression of the bHLH transcription factors Olig2 and neurogenin2 (Ngn2), respectively. Furthermore, we reveal that Ngn2-positive progenies express the T-box transcription factors Tbr2 and Tbr1, which are usually present during cortical and hippocampal glutamatergic neuronal differentiation. We also highlight a cell population expressing another bHLH transcription factor, neuroD1 (ND1). The ND1-positive cells are located in the SVZ-RMS axis and also co-express Tbr2, Tbr1 and neuroD2. The observations that these cells incorporate bromodeoxyuridine and express both doublecortin and polysialylated form of neural cell adhesion molecule suggest that they are newborn neurons. Finally, using an in vitro assay, we demonstrate that Ngn2 and ND1 equally and exclusively direct differentiation of Mash1-expressing precursors into calbindin-expressing and calretinin-expressing neurons, which are both neuronal subtypes normally found in the olfactory bulb. Taken together, our data illustrate that Ngn2, neuroD and Tbr transcription factors are involved in postnatal neurogenesis in the olfactory bulb.
机译：产后神经发生发生在两个脑区，海马区和脑室下区（SVZ）。已经详细描述了控制海马神经发生的转录级联反应。但是，嗅球神经发生的转录控制仍然没有很好的定位。在这项研究中，我们提供了有关控制产后嗅球神经发生的分子事件的见解。我们首先展示了沿着SVZ-侧流迁徙流（RMS）轴存在各种神经干细胞/祖细胞群，重点是那些表达基本螺旋-环-螺旋（bHLH）转录因子Mash1的种群。我们提供的证据表明，Mash1衍生的子代分别通过bHLH转录因子Olig2和Neurogenin2（Ngn2）的瞬时表达而产生少突胶质细胞和神经元前体。此外，我们发现Ngn2阳性子代表达T-box转录因子Tbr2和Tbr1，通常在皮质和海马谷氨酸能神经元分化过程中存在。我们还强调了表达另一种bHLH转录因子NeuroD1（ND1）的细胞群体。 ND1阳性细胞位于SVZ-RMS轴上，还共同表达Tbr2，Tbr1和NeuroD2。这些细胞掺入溴脱氧尿苷并表达神经细胞粘附分子的双皮质醇和多唾液酸化形式的观察结果表明它们是新生神经元。最后，使用体外测定法，我们证明Ngn2和ND1均等地和唯一地将表达Mash1的前体分化为表达calbindin和表达calretinin的神经元，它们都是嗅球中通常存在的神经元亚型。两者合计，我们的数据表明，Ngn2，neuroD和Tbr转录因子参与嗅球的产后神经发生。

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