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首页> 外文期刊>The European Journal of Neuroscience >Glutamatergic input is selectively increased in dorsal raphe subfield 5-HT neurons: role of morphology, topography and selective innervation.
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Glutamatergic input is selectively increased in dorsal raphe subfield 5-HT neurons: role of morphology, topography and selective innervation.

机译：谷氨酸能输入在背缝亚域5-HT神经元中选择性增加：形态，地形和选择性神经支配的作用。

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Characterization of glutamatergic input to dorsal raphe (DR) serotonin (5-HT) neurons is crucial for understanding how the glutamate and 5-HT systems interact in psychiatric disorders. Markers of glutamatergic terminals, vGlut1, 2 and 3, reflect inputs from specific forebrain and midbrain regions. Punctate staining of vGlut2 was homogeneous throughout the mouse DR whereas vGlut1 and vGlut3 puncta were less dense in the lateral wing (lwDR) compared with the ventromedial (vmDR) subregion. The distribution of glutamate terminals was consistent with the lower miniature excitatory postsynaptic current frequency found in the lwDR; however, it was not predictive of glutamatergic synaptic input with local activity intact, as spontaneous excitatory postsynaptic current (sEPSC) frequency was higher in the lwDR. We examined the morphology of recorded cells to determine if variations in dendrite structure contributed to differences in synaptic input. Although lwDR neurons had longer, more complex dendrites than vmDR neurons, glutamatergic input was not correlated with dendrite length in the lwDR, suggesting that dendrite length did not contribute to subregional differences in sEPSC frequency. Overall, glutamatergic input in the DR was the result of selective innervation of subpopulations of 5-HT neurons and was rooted in the topography of DR neurons and the activity of glutamate neurons located within the midbrain slice. Increased glutamatergic input to lwDR cells potentially synergizes with previously reported increased intrinsic excitability of lwDR cells to increase 5-HT output in lwDR target regions. Because the vmDR and lwDR are involved in unique circuits, subregional differences in glutamate modulation may result in diverse effects on 5-HT output in stress-related psychopathology.
机译：背缝（DR）血清素（5-HT）神经元的谷氨酸输入的特征对于了解谷氨酸和5-HT系统在精神疾病中的相互作用至关重要。谷氨酸能终末标志物vGlut1、2和3反映了特定前脑和中脑区域的输入。 vGlut2的点状染色在整个小鼠DR中均一，而vGlut1和vGlut3的点状点在侧翼（lwDR）中的密度低于腹膜（vmDR）子区域。谷氨酸末端的分布与在lwDR中发现的较低的微型兴奋性突触后电流频率一致;然而，由于lwDR中自发性兴奋性突触后电流（sEPSC）频率较高，因此无法预测具有局部活动的谷氨酸能突触输入。我们检查了记录的细胞的形态，以确定树突结构的变化是否导致突触输入的差异。尽管lwDR神经元比vmDR神经元具有更长，更复杂的树突，但谷氨酸能输入与lwDR中的树突长度无关，这表明树突长度对sEPSC频率的亚区域差异无贡献。总体而言，DR中的谷氨酸能输入是5-HT神经元亚群选择性神经支配的结果，其根源于DR神经元的地形和位于中脑片内的谷氨酸神经元的活性。向lwDR细胞增加的谷氨酸能输入可能与先前报道的lwDR细胞固​​有的兴奋性增加，从而增加lwDR目标区域的5-HT输出协同作用。由于vmDR和lwDR参与了独特的电路，因此谷氨酸调节的子区域差异可能会导致与压力相关的精神病理学对5-HT输出的多种影响。

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