...
  • 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics.
【24h】

Profiling of experience-regulated proteins in the songbird auditory forebrain using quantitative proteomics.

机译：使用定量蛋白质组学对鸣禽听觉前脑中的经验调节蛋白进行分析。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Auditory and perceptual processing of songs are required for a number of behaviors in songbirds such as vocal learning, territorial defense, mate selection and individual recognition. These neural processes are accompanied by increased expression of a few transcription factors, particularly in the caudomedial nidopallium (NCM), an auditory forebrain area believed to play a key role in auditory learning and song discrimination. However, these molecular changes are presumably part of a larger, yet uncharacterized, protein regulatory network. In order to gain further insight into this network, we performed two-dimensional differential in-gel expression (2D-DIGE) experiments, extensive protein quantification analyses, and tandem mass spectrometry in the NCM of adult songbirds hearing novel songs. A subset of proteins was selected for immunocytochemistry in NCM sections to confirm the 2D-DIGE findings and to provide additional quantitative and anatomical information. Using these methodologies, we found thatstimulation of freely behaving birds with conspecific songs did not significantly impact the NCM proteome 5 min after stimulus onset. However, following 1 and 3 h of stimulation, a significant number of proteins were consistently regulated in NCM. These proteins spanned a range of functional categories that included metabolic enzymes, cytoskeletal molecules, and proteins involved in neurotransmitter secretion and calcium binding. Our findings suggest that auditory processing of vocal communication signals in freely behaving songbirds triggers a cascade of protein regulatory events that are dynamically regulated through activity-dependent changes in calcium levels.
机译：歌曲中的许多行为都需要对歌曲进行听觉和感知处理，例如声音学习，领土防御，伴侣选择和个人识别。这些神经过程伴随着一些转录因子的表达增加，特别是在颅内侧膜上皮区（NCM）中，该区域被认为在听觉学习和歌曲识别中起关键作用。但是，这些分子变化可能是较大但尚未表征的蛋白质调节网络的一部分。为了进一步了解该网络，我们在听到新歌的成年鸣禽的NCM中进行了二维差分凝胶内表达（2D-DIGE）实验，广泛的蛋白质定量分析和串联质谱。选择了蛋白质的一个子集用于NCM切片中的免疫细胞化学，以确认2D-DIGE的发现并提供其他定量和解剖信息。使用这些方法，我们发现在刺激发生后5分钟，用特定的歌曲自由表现的鸟类的刺激不会显着影响NCM蛋白质组。但是，在刺激1和3小时后，NCM中大量蛋白质被一致地调节。这些蛋白质涵盖了一系列功能类别，包括代谢酶，细胞骨架分子以及与神经递质分泌和钙结合有关的蛋白质。我们的发现表明，在自由表现的鸣禽中对声音交流信号进行听觉处理会触发一系列蛋白质调节事件，这些事件通过钙水平的活动依赖性变化而动态调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号