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首页> 外文期刊>The European Journal of Neuroscience >Predominant expression of phospholipase Cbeta1 in telencephalic principal neurons and cerebellar interneurons, and its close association with related signaling molecules in somatodendritic neuronal elements.
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Predominant expression of phospholipase Cbeta1 in telencephalic principal neurons and cerebellar interneurons, and its close association with related signaling molecules in somatodendritic neuronal elements.

机译：磷脂酶Cbeta1在端脑主要神经元和小脑中间神经元中的主要表达，并与体树突状神经元中的相关信号分子密切相关。

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摘要

Upon activation of receptors coupled to the Gq subclass of G proteins, phospholipase C (PLC)beta hydrolyses membrane phospholipid to yield a pair of second messengers, inositol 1,4,5-trisphosphate and 1,2-diacylglycerol. Of four PLCbeta isoforms, PLCbeta1 is transcribed predominantly in the telencephalon and its gene inactivation in mice impairs metabotropic glutamate receptor- and muscarinic acetylcholine receptor-dependent hippocampal oscillations, endocannabinoid production in the hippocampus and barrel formation in the somatosensory cortex. Here we examined cellular and subcellular distributions of PLCbeta1 in adult mouse brains. In the telencephalon, high levels of PLCbeta1 were observed in principal neurons, including pyramidal cells in the cortex and hippocampus, granule cells and mossy cells in the dentate gyrus, and medium spiny neurons in the caudate-putamen, whereas most interneurons had low levels of or were negative for PLCbeta1 and, instead, expressed PLCbeta4. By immunofluorescence, tinyclusters of PLCbeta1 were distributed in somatodendritic compartments of principal neurons and positioned close to those of metabotropic glutamate receptor 5, muscarinic acetylcholine receptor M1 and diacylglycerol lipase-alpha, respectively. Immunoelectron microscopy revealed that PLCbeta1 was often associated with the smooth endoplasmic reticulum, cell membrane or postsynaptic density. In particular, it was highly accumulated at the perisynapse of dendritic spines forming asymmetrical synapses. In the cerebellum, PLCbeta1 was generally low but was enriched in axons and dendrites of basket cells. These results suggest that PLCbeta1 is the key effector in telencephalic principal neurons and cerebellar interneurons. Furthermore, the well-orchestrated molecular arrangement appears to be the anatomical basis for the specificity, efficiency and convergence of the neuronal phosphoinositide signaling system.
机译：激活与G蛋白的Gq亚类偶联的受体后，磷脂酶C（PLC）β水解膜磷脂，产生一对第二信使，即肌醇1,4,5-三磷酸酯和1,2-二酰基甘油。在四种PLCbeta亚型中，PLCbeta1主要在脑末梢转录，小鼠中的基因失活损害了代谢型谷氨酸受体和毒蕈碱型乙酰胆碱受体依赖性海马振荡，海马内源性大麻素生成以及体感皮层的桶状形成。在这里，我们检查了成年小鼠大脑中PLCbeta1的细胞和亚细胞分布。在端脑中，在主要神经元中观察到高水平的PLCbeta1，包括皮质和海马中的锥体细胞，齿状回中的颗粒细胞和苔藓细胞，以及尾状-丘脑中的中棘神经元，而大多数中神经元的水平较低。或对PLCbeta1阴性，而是表示PLCbeta4。通过免疫荧光，PLCbeta1的小簇分布在主要神经元的体状树突状区室中，并分别靠近代谢型谷氨酸受体5，毒蕈碱乙酰胆碱受体M1和二酰基甘油脂酶-α。免疫电子显微镜检查显示，PLCbeta1通常与平滑的内质网，细胞膜或突触后密度有关。特别地，它高度聚集在树突棘的突触周围，形成不对称突触。在小脑中，PLCbeta1通常较低，但富含篮子细胞的轴突和树突。这些结果表明PLCbeta1是端脑主要神经元和小脑中间神经元的关键效应器。此外，精心安排的分子排列似乎是神经元磷酸肌醇信号传导系统的特异性，效率和收敛性的解剖学基础。

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