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首页> 外文期刊>The European Journal of Neuroscience >Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation.
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Characterization of NO-sensitive guanylyl cyclase: expression in an identified interneuron involved in NO-cGMP-dependent memory formation.

机译：NO敏感的鸟苷酸环化酶的表征：在已确定的参与NO-cGMP依赖性记忆形成的中间神经元中表达。

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摘要

In a number of neuronal models of learning signalling by endogenous nitric oxide (NO), produced by the enzyme NO synthase (NOS), is essential for the formation of long-term memory (LTM). For example, in the molluscan model system Lymnaea, NO is required for LTM formation in the first few hours after one-trial reward conditioning. Furthermore, conditioning leads to transient up-regulation of the NOS gene in identified modulatory neurons, the cerebral giant cells (CGCs), which are known to be involved in LTM formation. In Lymnaea nothing is known however about the structure and localization of the major receptor for NO, the soluble guanylyl cyclase (sGC). Here we report on the cloning and characterization of both alpha and beta subunits of NO-sensitive sGC and show that they are coexpressed in the CGCs. Furthermore, our electrophysiological experiments on isolated CGCs show that these neurons respond to NO by generating a prolonged depolarization of the membrane potential. Moreover, we demonstrate that this depolarization is blocked by ODQ, supporting our hypothesis that it is mediated by sGC.
机译：在由NO合酶（NOS）产生的内源性一氧化氮（NO）学习信号的许多神经元模型中，长期记忆（LTM）的形成至关重要。例如，在软体动物模型系统Lymnaea中，在一次试验奖励条件调整后的最初几个小时内，LTM的形成不需要NO。此外，调节导致识别的调节性神经元即大脑巨细胞（CGC）中NOS基因的瞬时上调，已知该神经元参与了LTM的形成。然而，在Lymnaea中，关于NO的主要受体，可溶性鸟苷酸环化酶（sGC）的结构和定位还一无所知。在这里，我们报告的NO敏感sGC的α和β亚基的克隆和表征，并表明它们在CGC中共表达。此外，我们对离体CGC的电生理实验表明，这些神经元通过产生长时间的膜电位去极化来响应NO。此外，我们证明了这种去极化作用被ODQ阻断，支持了我们的假设，即它是由sGC介导的。

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