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首页> 外文期刊>The European Journal of Neuroscience >Intrinsic activity and positive feedback in motor circuits in organotypic spinal cord slice cultures.
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Intrinsic activity and positive feedback in motor circuits in organotypic spinal cord slice cultures.

机译：器官型脊髓切片培养物中运动回路的内在活性和正反馈。

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In co-cultures of embryonic rat spinal cord slices and skeletal muscle, spinal motoneurons innervate muscle fibres and drive muscle contractions. However, multi-electrode array (MEA) recordings show that muscle contractions often appear in the absence of population activity in the spinal cord networks. Such uncorrelated muscle activity persists when the population bursts in the neuronal networks are prevented by un-coupling the network with the glutamatergic antagonists CNQX and D-APV. By contrast, the uncorrelated muscle activity is fully suppressed by the muscular nicotinic antagonist D-tubocurarine. Together, these findings confirm the previous finding that motoneurons drive muscle fibres in this preparation and suggest that they are intrinsically spiking in the absence of synaptic input. Intracellular recordings from spinal neurons support this suggestion. Analysing the correlated muscle activity, we found that in 15% of the population bursts, muscle activity appears at the beginning or before neuronal activity, suggesting that in these cases motoneurons initiate the population activity. Both the total number of population bursts and the percentage of such bursts that are initiated by muscle activity are reduced by a block of nicotinic receptors. Uncorrelated muscle and neuronal activity is reduced by the gap junction blocker carbenoxolone, suggesting that electrical coupling is involved in the generation of this activity. Together, these findings suggest that intrinsic firing of motoneurons may contribute to the activation of population bursts through cholinergic positive feedback loops in cultured spinal networks.
机译：在胚胎大鼠脊髓切片和骨骼肌的共培养中，脊髓运动神经元支配肌纤维并驱动肌肉收缩。但是，多电极阵列（MEA）记录显示，肌肉收缩通常在脊髓网络中没有种群活动的情况下出现。当通过使神经网络与谷氨酸能拮抗剂CNQX和D-APV脱钩而阻止神经网络中的群体爆发时，这种不相关的肌肉活动将继续存在。相反，不相关的肌肉活动被肌肉烟碱拮抗剂D-微管尿素完全抑制。总之，这些发现证实了以前的发现，即运动神经元在这种制剂中驱动肌肉纤维，并表明它们在没有突触输入的情况下本质上是尖峰的。脊髓神经元的细胞内记录支持这一建议。通过分析相关的肌肉活动，我们发现在15％的人群爆发中，肌肉活动出现在神经元活动的开始或之前，这表明在这种情况下，运动神经元启动了人群活动。一组烟碱受体减少了群体爆发的总数和这种由肌肉活动引发的爆发的百分比。间隙连接阻滞剂羧苄隆减少了不相关的肌肉和神经元活动，表明电耦合参与了这种活动的产生。总之，这些发现表明，运动神经元的内在激发可能通过培养的脊柱网络中的胆碱能正反馈回路来促进种群爆发的激活。

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