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首页> 外文期刊>The European Journal of Neuroscience >Visualizing jet lag in the mouse suprachiasmatic nucleus and peripheral circadian timing system.
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Visualizing jet lag in the mouse suprachiasmatic nucleus and peripheral circadian timing system.

机译：可视化小鼠视交叉上核和周围昼夜节律计时系统中的时差。

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Circadian rhythms regulate most physiological processes. Adjustments to circadian time, called phase shifts, are necessary following international travel and on a more frequent basis for individuals who work non-traditional schedules such as rotating shifts. As the disruption that results from frequent phase shifts is deleterious to both animals and humans, we sought to better understand the kinetics of resynchronization of the mouse circadian system to one of the most disruptive phase shifts, a 6-h phase advance. Mice bearing a luciferase reporter gene for mPer2 were subjected to a 6-h advance of the light cycle and molecular rhythms in suprachiasmatic nuclei (SCN), thymus, spleen, lung and esophagus were measured periodically for 2 weeks following the shift. For the SCN, the master pacemaker in the brain, we employed high-resolution imaging of the brain slice to describe the resynchronization of rhythms in single SCN neurons during adjustment to the new light cycle. We observed significant differences in shifting kinetics among mice, among organs such as the spleen and lung, and importantly among neurons in the SCN. The phase distribution among all Period2-expressing SCN neurons widened on the day following a shift of the light cycle, which was partially due to cells in the ventral SCN exhibiting a larger initial phase shift than cells in the dorsal SCN. There was no clear delineation of ventral and dorsal regions, however, as the SCN appear to have a population of fast-shifting cells whose anatomical distribution is organized in a ventral-dorsal gradient. Full resynchronization of the SCN and peripheral timing system, as measured by a circadian reporter gene, did not occur until after 8 days in the advanced light cycle.
机译：昼夜节律调节大多数生理过程。在国际旅行之后，有必要对昼夜节律时间进行调整，称为相移，对于工作非传统时间表（例如轮班）的个人，更经常地进行调整。由于频繁相移引起的破坏对动物和人类均有害，因此我们试图更好地了解小鼠昼夜节律系统重新同步至最具破坏性的相移之一（6小时相移）的动力学。携带带有mPer2荧光素酶报道基因的小鼠进行光循环6小时，并在转移后2周内定期测量上交叉眼上核（SCN），胸腺，脾脏，肺和食道的分子节律。对于SCN，即大脑中的主要起搏器，我们采用了脑切片的高分辨率成像来描述在适应新的光周期过程中单个SCN神经元的节律重新同步。我们观察到小鼠之间，脾脏和肺等器官之间，尤其是SCN中的神经元之间，在转移动力学上存在显着差异。在光周期改变后的一天，所有表达Period2的SCN神经元之间的相分布变宽，部分原因是腹侧SCN中的细胞比背侧SCN中的细胞显示出更大的初始相移。然而，由于SCN似乎有一群快速移动的细胞，其解剖学分布以腹背梯度排列，因此尚无清晰的腹侧和背侧区域的轮廓。通过昼夜节律报道基因测得的SCN和外围计时系统完全重新同步，直到高级光照周期中的8天后才发生。

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