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首页> 外文期刊>The European Journal of Neuroscience >Thyroid hormone regulates TAG-1 expression in the developing rat brain.
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Thyroid hormone regulates TAG-1 expression in the developing rat brain.

机译：甲状腺激素调节发育中的大鼠大脑中TAG-1的表达。

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摘要

TAG-1 is a member of the immunoglobulin superfamily of cell adhesion molecules thought to play important roles in neuronal differentiation and the establishment of connectivity during brain development. Because these are processes also affected by hypothyroidism, we studied the effects of thyroid hormone deprivation and administration on TAG-1 expression in the developing rat brain. By in situ hybridization, immunohistochemistry and Western blotting we found that TAG-1 RNA and protein levels are upregulated in the hypothyroid brain. From embryonic day 20 to postnatal day (P) 15, elevated TAG-1 RNA was found in several areas including the cerebral cortex, hippocampus and olfactory bulb. In agreement with this, TAG-1 protein was overexpressed in the major fibre tracts arising from these structures, including the corpus callosum, anterior and hippocampal commissures and lateral olfactory tract. A similar overexpression of TAG-1 by hypothyroidism was detected in the cerebellum, but starting only at P15. In all cases, elevation of TAG-1 RNA and protein expression could be reversed by thyroid hormone treatment. These results show that the deregulation of TAG-1 might contribute to the alterations caused by the lack of thyroid hormone during brain development.
机译：TAG-1是细胞粘附分子免疫球蛋白超家族的成员，被认为在大脑发育过程中在神经元分化和建立连接方面起重要作用。由于这些过程也受到甲状腺功能减退的影响，因此我们研究了甲状腺激素剥夺和给药对发育中大鼠大脑中TAG-1表达的影响。通过原位杂交，免疫组化和Western印迹，我们发现甲状腺功能减退性大脑中的TAG-1 RNA和蛋白质水平上调。从胚胎第20天到产后第15天，在大脑皮层，海马和嗅球等多个区域发现了TAG-1 RNA升高。与此相符的是，TAG-1蛋白在由这些结构引起的主要纤维束中过表达，这些组织包括call体，前，海马连合和外侧嗅道。在小脑中检测到甲状腺机能减退引起的TAG-1类似过表达，但仅在P15开始。在所有情况下，甲状腺激素治疗均可逆转TAG-1 RNA和蛋白质表达的升高。这些结果表明，TAG-1的失调可能有助于大脑发育过程中甲状腺激素缺乏引起的改变。

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