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首页> 外文期刊>The European Journal of Neuroscience >SK channel blockade promotes burst firing in dorsal raphe serotonergic neurons.
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SK channel blockade promotes burst firing in dorsal raphe serotonergic neurons.

机译：SK通道阻滞促进背侧缝血清素能神经元的爆发放电。

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摘要

Previous in vivo studies have shown that blockade of small-conductance Ca(2+)-activated potassium (SK) channels enhances burst firing in dopaminergic neurons. As bursting has been found to be physiologically relevant for the synaptic release of serotonin (5-HT), we investigated the possible role of SK channels in the control of this firing pattern in 5-HT neurons of the dorsal raphe nucleus. In these cells, bursts are usually composed of doublets consisting of action potentials separated by a small interval (< 20 ms). Both in vivo and in vitro extracellular recordings were performed, using anesthetized rats and rat brain slices, respectively. In vivo, the specific SK blocker UCL 1684 (200 microm) iontophoresed onto presumed 5-HT neurons significantly increased the production of bursts in 13 out of 25 cells. Furthermore, the effect of UCL 1684 persisted in the presence of both the GABA(A) antagonist SR 95531 (10 mm) and the GABA(B) antagonist CGP 35348 (10 mm), whereas these agents by themselves did notsignificantly influence the neuronal firing pattern. In vitro, bath superfusion of the SK channel blocker apamin (300 nm) induced bursting in only three out of 18 neurons, although it increased the coefficient of variation of the interspike intervals in all the other cells. Our results suggest that SK channel blockade promotes bursting activity in 5-HT neurons via a direct action. An input which is present only in vivo seems to be important for the induction of this firing pattern in these cells.
机译：先前的体内研究表明，小电导Ca（2+）激活的钾（SK）通道的封锁会增强多巴胺能神经元的爆发放电。由于已发现爆发与5-羟色胺（5-HT）的突触释放在生理上有关，因此我们研究了SK通道在控制背ra核5-HT神经元中这种触发方式中的可能作用。在这些单元格中，突发通常由双峰构成，双峰由以小间隔（<20 ms）隔开的动作电位组成。分别使用麻醉的大鼠和大鼠脑切片进行体内和体外细胞外记录。在体内，离子电渗入假定的5-HT神经元上的特异性SK阻滞剂UCL 1684（200微米）显着增加了25个细胞中有13个细胞爆发的产生。此外，在同时存在GABA（A）拮抗剂SR 95531（10 mm）和GABA（B）拮抗剂CGP 35348（10 mm）的情况下，UCL 1684的作用持续存在，而这些药物本身对神经元放电没有明显影响。模式。在体外，SK通道阻滞剂apamin（300 nm）的浴液灌注诱导了18个神经元中只有3个的爆发，尽管它增加了所有其他细胞中突突间隔的变异系数。我们的结果表明，SK通道阻滞通过直接作用促进5-HT神经元的爆发活动。仅在体内存在的输入似乎对于在这些细胞中诱导这种放电模式很重要。

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