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首页> 外文期刊>The European Journal of Neuroscience >Network activity and spike discharge oscillations in cortical slice cultures from neonatal rat.
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Network activity and spike discharge oscillations in cortical slice cultures from neonatal rat.

机译：新生大鼠皮质切片培养物中的网络活动和峰值放电振荡。

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摘要

Network bursts and oscillations are forms of spontaneous activity in cortical circuits that have been described in vivo and in vitro. Searching for mechanisms involved in their generation, we investigated the collective network activity and spike discharge oscillations in cortical slice cultures of neonatal rats, combining multielectrode arrays with patch clamp recordings from individual neurons. The majority of these cultures showed spontaneous collective network activity [population bursts (PBs)] that could be described as neuronal avalanches. The largest of these PBs were followed by fast spike discharge oscillations in the beta to theta range, and sometimes additional repetitive PBs, together forming seizure-like episodes. During such episodes, all neurons showed sustained depolarization with increased spike rates. However, whereas regular-spiking (RS) and fast-spiking (FS) neurons fired during the PBs, only the FS neurons fired during the fast oscillations. Blockade of N-methyl-d-aspartate receptors reduced the depolarization and suppressed both the increased FS neuron firing and the oscillations. To investigate the generation of PBs, we studied the network responses to electrical stimulation. For most of the stimulation sites, the relationship between the stimulated inputs and the evoked PBs was linear. From a few stimulation sites, however, large PBs could be evoked with small inputs, indicating the activation of hub circuits. Taken together, our findings suggests that the oscillations originate from recurrent inhibition in local networks of depolarized inhibitory FS interneurons, whereas the PBs originate from recurrent excitation in networks of RS and FS neurons that is initiated in hub circuits.
机译：网络爆发和振荡是皮质回路中自发活动的形式，已在体内和体外进行了描述。为寻找参与其生成的机制，我们研究了新生大鼠皮质切片培养物中的集体网络活动和峰值放电振荡，将多电极阵列与单个神经元的膜片钳记录相结合。这些文化的大多数表现出自发的集体网络活动[人口爆发（PBs）]，可以描述为神经元雪崩。这些PB中最大的是β至theta范围内的快速尖峰放电振荡，有时还有其他重复性PB，一起形成癫痫样发作。在此类发作期间，所有神经元均表现出持续的去极化作用，并伴随着尖峰频率增加。但是，尽管在PB期间触发了常规加长（RS）和快速加急（FS）的神经元，但在快速振荡期间仅触发了FS神经元。 N-甲基-d-天冬氨酸受体的阻滞减少了去极化，并抑制了FS神经元放电的增加和振荡。为了研究PB的生成，我们研究了网络对电刺激的响应。对于大多数刺激部位，刺激输入和诱发PB之间的关系是线性的。但是，从几个刺激点开始，大的PB可能由小的输入引起，这表明集线器电路已激活。综上所述，我们的发现表明，振荡起因于去极化抑制性FS中间神经元的局部网络中的递归抑制，而PB则起源于集线器回路中启动的RS和FS神经元网络的递归激励。

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