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首页> 外文期刊>The European Journal of Neuroscience >Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin - a postembedding immunogold study in the healthy rat brain
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Rare contacts between synapses and microglial processes containing high levels of Iba1 and actin - a postembedding immunogold study in the healthy rat brain

机译：突触与含有高水平Iba1和肌动蛋白的小神经胶质突触之间的罕见接触-在健康大鼠大脑中进行的包埋后免疫金研究

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Although microglia is recognised as the cell-mediating innate immunity in the brain, emerging evidence suggests a role of microglia in synaptic communication and modulation. The ability of microglia to move in the neuropil and contact synapses is crucial for such a function. However, the frequency of microglial contact with synapses is not known. Microglia motility is regulated by actin polymerisation and its interaction with ionising calcium-binding adaptor protein 1 (Iba1). In order to move and make contact with synapses, delicate microglial processes should contain high levels of actin and Iba1. To study this we refined an electron microscopic postembedding immunogold method enabling us to identify and quantitatively study different microglial constituents in intact brain tissue. We show that Iba1 and actin were colocalised at high densities in delicate processes in the rat frontal cortex, and that these delicate processes of microglia contact synaptic elements. About 3.5% of the synapses received direct contact from microglia. There was a marked inverse correlation between the densities of Iba1/actin gold particles and the area of the microglial processes, suggesting that the most delicate processes possess the machinery to provide movement in the neuropil. The low frequency of microglia interaction with synaptic elements suggests that microglia have a limited role in overall regulation of synaptic activity.
机译：尽管小胶质细胞被认为是大脑中介导细胞的先天免疫，但新出现的证据表明小胶质细胞在突触通讯和调节中的作用。小胶质细胞在神经纤维中移动和接触突触的能力对于这种功能至关重要。但是，小胶质细胞与突触接触的频率未知。小胶质细胞的运动受肌动蛋白聚合及其与电离钙结合衔接蛋白1（Iba1）的相互作用所调节。为了移动并与突触接触，精细的小胶质细胞过程应包含高水平的肌动蛋白和Iba1。为了对此进行研究，我们改进了电子显微镜后包埋免疫金方法，使我们能够识别和定量研究完整脑组织中不同的小胶质细胞成分。我们显示，Iba1和肌动蛋白在大鼠额叶皮层的微妙过程中以高密度共定位，并且这些小胶质细胞的微妙过程接触突触元件。约3.5％的突触直接与小胶质细胞接触。 Iba1 /肌动蛋白金颗粒的密度与小胶质细胞突区域之间存在显着的负相关关系，这表明最微妙的过程具有在神经纤维中提供运动的机制。小胶质细胞与突触元件相互作用的频率低表明，小胶质细胞在突触活性的整体调节中作用有限。

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