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首页> 外文期刊>The European Journal of Neuroscience >Estrogen and exercise interact to regulate brain-derived neurotrophic factor mRNA and protein expression in the hippocampus.
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Estrogen and exercise interact to regulate brain-derived neurotrophic factor mRNA and protein expression in the hippocampus.

机译：雌激素和运动相互作用以调节海马中脑源性神经营养因子的mRNA和蛋白表达。

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We investigated the possibility that estrogen and exercise interact in the hippocampus and regulate brain-derived neurotrophic factor (BDNF), a molecule increasingly recognized for its role in plasticity and neuron function. An important aspect of this study is to examine the effect of different time intervals between estrogen loss and estrogen replacement intervention. We demonstrate that in the intact female rat, physical activity increases hippocampal BDNF mRNA and protein levels. However, the exercise effect on BDNF up-regulation is reduced in the absence of estrogen, in a time-dependent manner. In addition, voluntary activity itself is stimulated by the presence of estrogen. In exercising animals, estrogen deprivation reduced voluntary activity levels, while estrogen replacement restored activity to normal levels. In sedentary animals, estrogen deprivation (ovariectomy) decreased baseline BDNF mRNA and protein, which were restored by estrogen replacement. Despite reduced activity levels in the ovariectomized condition, exercise increased BDNF mRNA levels in the hippocampus after short-term (3 weeks) estrogen deprivation. However, long-term estrogen-deprivation blunted the exercise effect. After 7 weeks of estrogen deprivation, exercise alone no longer affected either BDNF mRNA or protein levels. However, exercise in combination with long-term estrogen replacement increased BDNF protein above the effects of estrogen replacement alone. Interestingly, protein levels across all conditions correlated most closely with mRNA levels in the dentate gyrus, suggesting that expression of mRNA in this hippocampal region may be the major contributor to the hippocampal BDNF protein pool. The interaction of estrogen, physical activity and hippocampal BDNF is likely to be an important issue for maintenance of brain health, plasticity and general well-being, particularly in women.
机译：我们调查了雌激素和运动在海马中相互作用并调节脑源性神经营养因子（BDNF）的可能性，BDNF是一种分子，在其可塑性和神经元功能中得到越来越多的认可。这项研究的重要方面是检查雌激素流失和雌激素替代干预之间不同时间间隔的影响。我们证明，在完整的雌性大鼠中，体育锻炼会增加海马BDNF mRNA和蛋白质水平。但是，在缺乏雌激素的情况下，运动对BDNF上调的作用会以时间依赖的方式降低。另外，雌激素的存在刺激了自愿活动本身。在锻炼动物时，雌激素剥夺降低了自愿活动水平，而雌激素替代使活动恢复到正常水平。在久坐的动物中，雌激素剥夺（卵巢切除术）会降低基线BDNF mRNA和蛋白，这些蛋白可通过雌激素替代而恢复。尽管在去卵巢条件下活动水平降低，但短期（3周）雌激素剥夺后，运动会使海马中的BDNF mRNA水平升高。然而，长期的雌激素剥夺削弱了运动效果。雌激素剥夺7周后，单独运动不再影响BDNF mRNA或蛋白质水平。但是，结合长期雌激素替代运动可增加BDNF蛋白，使其超过单独的雌激素替代作用。有趣的是，所有条件下的蛋白质水平与齿状回中的mRNA水平最密切相关，这表明该海马区域中mRNA的表达可能是海马BDNF蛋白库的主要贡献者。雌激素，身体活动和海马BDNF的相互作用可能是维持脑部健康，可塑性和总体健康的重要问题，特别是在女性中。

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