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首页> 外文期刊>The European Journal of Neuroscience >Cytochemical and cytological properties of perineuronal oligodendrocytes in the mouse cortex.
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Cytochemical and cytological properties of perineuronal oligodendrocytes in the mouse cortex.

机译：小鼠皮质神经周围神经少突胶质细胞的细胞化学和细胞学特性。

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摘要

Neuronal cell bodies are associated with glial cells collectively referred to as perineuronal satellite cells. One such satellite cell is the perineuronal oligodendrocyte, which is unmyelinating oligodendrocytes attaching to large neurons in various neural regions. However, little is known about their cellular characteristics and function. In this study, we identified perineuronal oligodendrocytes as 2',3'-cyclic nucleotide 3'-phosphodiesterase-positive cells attaching to neuronal perikarya immunostained for microtubule-associated protein 2, and examined their cytochemical and cytological properties in the mouse cerebral cortex. 2',3'-Cyclic nucleotide 3'-phosphodiesterase-positive perineuronal oligodendrocytes were immunonegative to representative glial markers for astrocytes (brain-type lipid binding protein and glial fibrillary acidic protein), microglia (Iba-1) and NG2(+) glia. However, almost all perineuronal oligodendrocytes expressed glia-specific or glia-enriched metabolic enzymes, i.e. the creatine synthetic enzyme S-adenosylmethionine:guanidinoacetate N-methyltransferase and L-serine biosynthetic enzyme 3-phosphoglycerate dehydrogenase. As to molecules participating in the glutamate-glutamine cycle, none of the perineuronal oligodendrocytes expressed the plasmalemmal glutamate transporters GLAST and GLT-1, although nearly half of the perineuronal oligodendrocytes were immunopositive for glutamine synthetase. Cytologically, perineuronal oligodendrocytes were mainly distributed in deep cortical layers (layers IV-VI), and attached directly and tightly to neuronal cell bodies, making a long concave impression to the contacting neurons. Interestingly, they attached more to glutamatergic principal neurons than to GABAergic interneurons, and this became evident at postnatal day 14, when the cerebral cortex develops and maturates. These cytochemical and cytological properties suggest that perineuronal oligodendrocytes are so differentiated as to fulfill metabolic support to the associating principal cortical neurons, rather than to regulate their synaptic transmission.
机译：神经元细胞体与神经胶质细胞相关联，神经胶质细胞统称为神经周围神经卫星细胞。一种这样的卫星细胞是神经周围神经少突胶质细胞，它是无髓鞘的少突胶质细胞，附着在各个神经区域的大神经元上。但是，关于它们的细胞特征和功能知之甚少。在这项研究中，我们确定了神经周围少突胶质细胞为2'，3'-环核苷酸3'-磷酸二酯酶阳性细胞，该细胞附着于免疫微管相关蛋白2染色的神经元周核上，并检查了它们在小鼠大脑皮层中的细胞化学和细胞学特性。 2'，3'-环状核苷酸3'-磷酸二酯酶阳性的神经周围少突胶质细胞对星形胶质细胞（脑型脂质结合蛋白和神经胶质纤维酸性蛋白），小胶质细胞（Iba-1）和NG2（+）胶质细胞的代表性胶质标记免疫阴性。但是，几乎所有的神经周围神经少突胶质细胞都表达神经胶质特异或神经胶质丰富的代谢酶，即肌酸合成酶S-腺苷甲硫氨酸：胍基乙酸酯N-甲基转移酶和L-丝氨酸生物合成酶3-磷酸甘油酸脱氢酶。至于参与谷氨酸-谷氨酰胺循环的分子，虽然将近一半的神经外少突胶质细胞对谷氨酰胺合成酶免疫阳性，但神经周围的少突胶质细胞都没有表达血浆中的谷氨酸转运蛋白GLAST和GLT-1。在细胞学上，神经周围神经少突胶质细胞主要分布在深层皮质层（第IV-VI层），并直接紧密地附着在神经元细胞体上，给接触的神经元留下长的凹痕。有趣的是，它们对谷氨酸能主神经元的附着比对GABA能神经元的附着更多，这在出生后第14天大脑皮层发育并成熟时变得明显。这些细胞化学和细胞学性质表明，神经周少突胶质细胞如此分化，以实现对相关的主要皮层神经元的代谢支持，而不是调节其突触传递。

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