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首页> 外文期刊>The European Journal of Neuroscience >Electrophysiological analysis of suprachiasmatic nucleus projections to the ventrolateral preoptic area in the rat.
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Electrophysiological analysis of suprachiasmatic nucleus projections to the ventrolateral preoptic area in the rat.

机译：大鼠视交叉上核投射到腹外侧前视区的电生理分析。

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摘要

The circadian pacemaker housed in the suprachiasmatic nucleus (SCN) synchronizes daily sleep-wake cycles, presumably by modulating the sleep-wake regulatory system, including ventrolateral preoptic area (VLPO) neurons. We used whole-cell patch-clamp recording to study the projections from the SCN to the VLPO in horizontal slices of rat hypothalamus. Single-pulse stimulation of the SCN region elicited postsynaptic currents (PSCs) in 20 of 66 neurons (30%) recorded within the VLPO region as verified by intracellular biocytin labelling. At a holding potential of -60 mV, the evoked PSCs had an amplitude of 17.6 +/- 3.2 pA (SEM) and a latency of 6.3 +/- 0.5 ms (n = 10). There was a trend for simple excitatory postsynaptic currents (EPSCs) to be evoked in the VLPO cluster, simple inhibitory postsynaptic currents (IPSCs) in the extended VLPO, and a combination of EPSCs and IPSCs in both regions. IPSCs were blocked reversibly by bicuculline (10 microm, n = 11). In both the presence and absence of bicuculline, EPSCs had fast and slow components that were blocked by 6,7-dinitroquinoxaline-2,3-dione (DNQX; 10 microm; n = 7), and (+/-)3-(2-carboxypiperazin-4-yl)-propyl-1-phosphonic acid (CPP; 10 microm, n = 6), respectively. Reversal potentials for the evoked IPSCs and EPSCs were consistent with mediation via GABAA and ionotropic glutamate receptors, respectively. These results suggest that the SCN region provides both inhibitory and excitatory inputs to single VLPO neurons, which are mediated, respectively, by GABAA receptors and by both non-NMDA and NMDA glutamate receptors. These projections may play important roles in conveying circadian input to systems in the preoptic area that regulate sleep and waking.
机译：安置在视交叉上核（SCN）中的昼夜节律起搏器可以通过调节睡眠-唤醒调节系统（包括腹外侧视前区（VLPO）神经元）来同步每天的睡眠-唤醒周期。我们使用全细胞膜片钳记录来研究大鼠下丘脑水平切片中从SCN到VLPO的投影。单脉冲刺激SCN区域会在VLPO区域内记录的66个神经元中的20个（30％）中引起突触后电流（PSC），这已通过细胞内生物素标记证明。在-60 mV的保持电势下，诱发的PSC的幅度为17.6 +/- 3.2 pA（SEM），潜伏期为6.3 +/- 0.5 ms（n = 10）。在VLPO簇中会出现简单的兴奋性突触后电流（EPSC），在扩展的VLPO中会出现简单的抑制性突触后电流（IPSC），并且在两个区域中都有EPSC和IPSC的组合。 IPSCs被双小分子（10微米，n = 11）可逆地阻断。在存在和不存在双小分子的情况下，EPSC的快和慢成分均被6,7-二硝基喹喔啉-2,3-二酮（DNQX; 10 microm; n = 7）和（+/-）3-（ 2-羧基哌嗪-4-基）-丙基-1-膦酸（CPP; 10微米，n = 6）。诱发的IPSC和EPSC的逆转电位分别与通过GABAA和离子型谷氨酸受体的介导一致。这些结果表明，SCN区为单个VLPO神经元提供了抑制性和兴奋性输入，分别由GABAA受体以及非NMDA和NMDA谷氨酸受体介导。这些预测可能在将昼夜节律输入传递到视前区调节睡眠和醒来的系统中起重要作用。

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