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首页> 外文期刊>The European Journal of Neuroscience >The speeding of EPSC kinetics during maturation of a central synapse.
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The speeding of EPSC kinetics during maturation of a central synapse.

机译：中央突触成熟期间EPSC动力学的加快。

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Several factors contribute to the shape of excitatory postsynaptic currents (EPSCs) in CNS neurons, among them the kinetics of presynaptic release, transmitter clearance, and the properties and distribution of postsynaptic receptors. The decays of AMPA receptor-mediated EPSCs at rat cerebellar mossy fibre-granule cell (MF-gc) synapses follow a bi-exponential time-course. The fast component dominates the decay, accounting for 84-94% of the peak amplitude. Here we show that both components of decay, and also the risetimes, became faster during postnatal maturation. At adult, but not immature, synapses, the risetimes and decays of evoked multiquantal EPSCs were similar to those of monoquantal miniature (m)EPSCs. The faster risetimes at mature synapses reflected increased synchrony of multivesicular release, whereas the faster decays appeared to reflect changes in the properties of postsynaptic receptors. Inhibition of glutamate uptake was without effect on evoked EPSCs at both ages. Furthermore, after slowing receptor desensitization with cyclothiazide, the EPSCs at mature synapses decayed as slowly as EPSCs at immature synapses, suggesting that faster glutamate clearance does not account for the developmental speeding of EPSC decay. Our results support previous conclusions that glutamate clearance and receptor deactivation are important determinants of the fast decay component at immature synapses. Desensitization becomes increasingly important during development and plays a major role in shaping EPSC decay at mature synapses.
机译：几个因素有助于中枢神经系统神经元的兴奋性突触后电流（EPSCs）的形状，其中包括突触前释放的动力学，递质清除以及突触后受体的性质和分布。 AMPA受体介导的EPSC在大鼠小脑苔藓纤维-颗粒细胞（MF-gc）突触处的衰减遵循双指数时间过程。快速分量在衰减中占主导地位，占峰值幅度的84-94％。在这里，我们显示了衰老的两个组成部分以及上升时间在产后成熟过程中都变得更快。在成年但不是未成熟的突触中，诱发的多量子EPSC的上升时间和衰减与单量子微型（m）EPSC相似。在成熟突触中更快的上升时间反映了多囊泡释放的同步性增加，而更快的衰减似乎反映了突触后受体特性的变化。在两个年龄段，抑制谷氨酸的摄取均对诱发的EPSC没有影响。此外，在用环噻嗪减缓受体脱敏作用后，成熟突触处的EPSC衰变与未成熟突触处的EPSC一样缓慢，这表明更快的谷氨酸清除率不能解释EPSC衰变的发展速度。我们的结果支持先前的结论，即谷氨酸清除和受体失活是未成熟突触中快速衰变成分的重要决定因素。脱敏在发育过程中变得越来越重要，并且在塑造成熟突触的EPSC衰减中起主要作用。

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