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首页> 外文期刊>The European Journal of Neuroscience >Sleep deprivation modulates brain mRNAs encoding genes of glycogen metabolism.
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Sleep deprivation modulates brain mRNAs encoding genes of glycogen metabolism.

机译：睡眠剥夺可调节编码糖原代谢基因的大脑mRNA。

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摘要

Replenishment of brain glycogen stores depleted during waking has been suggested to constitute one of the functions of sleep [Benington, J. H. & Heller H. C. (1995) Prog. Neurobiol., 45, 347]. We have tested the hypothesis that the level of expression of enzymes involved in glycogen metabolism could undergo variations throughout the sleep-waking or rest-activity cycle, and after 6 h of 'gentle' total sleep deprivation in mice. Specifically, we determined the variations in mRNAs coding for protein targeting to glycogen (PTG), glycogen synthase and glycogen phosphorylase, all considered as key regulators of glycogen metabolism. Glycogen synthase and glycogen phosphorylase mRNAs exhibited significant variations throughout the light-dark cycle with a maximum at the middle of the light period and a minimum at the middle of the dark period. Following sleep deprivation, a two-fold increase in PTG mRNA and a decrease of mRNAs encoding glycogen synthase and glycogen phosphorylase were observed. These transcriptional events have functional consequences as the activity of glycogen synthase was increased 2.5-fold indicating a stimulating effect of sleep deprivation on glycogen synthesis. These results indicate that (i) expression of genes related to brain glycogen metabolism exhibit variations throughout the sleep-waking or rest-activity cycle and (ii) given the almost selective localization of glycogen to astrocytes, these cells might participate in the regulation of sleep.
机译：已经提出，在清醒期间消耗掉的脑糖原储备的补充构成睡眠的功能之一[Benington，J.H。和Heller H.C.（1995）Prog.Natl.Acad.Sci.USA，95：1877-1099]。神经生物学杂志，第45卷，第347页]。我们已经测试了一种假设，即参与糖原代谢的酶的表达水平可能在整个睡眠-觉醒或休息-活动周期中以及在小鼠“温和”的总睡眠剥夺6小时后发生变化。具体来说，我们确定了编码针对糖原（PTG），糖原合酶和糖原磷酸化酶的蛋白质的mRNA的变异，这些均被视为糖原代谢的关键调节因子。糖原合酶和糖原磷酸化酶mRNA在整个明暗循环中表现出明显的变化，在亮周期的中间出现最大值，在暗周期的中间出现最小值。睡眠剥夺后，观察到PTG mRNA的增加两倍，编码糖原合酶和糖原磷酸化酶的mRNA减少。这些转录事件具有功能性后果，因为糖原合酶的活性增加了2.5倍，表明睡眠剥夺对糖原合成有刺激作用。这些结果表明：（i）与脑糖原代谢相关的基因的表达在整个睡眠-觉醒或休息-活动周期中均表现出变化，并且（ii）鉴于糖原几乎选择性地定位于星形胶质细胞，这些细胞可能参与了睡眠的调节。

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