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首页> 外文期刊>The European Journal of Neuroscience >Expression of RGS2, RGS4 and RGS7 in the developing postnatal brain.
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Expression of RGS2, RGS4 and RGS7 in the developing postnatal brain.

机译：RGS2，RGS4和RGS7在发育中的产后大脑中的表达。

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The abundant expression of RGS (regulator of G-protein signalling) proteins in neurons, together with their modulatory function on G-protein-dependent neurotransmission, provides the basis for cellular adaptation to sensory inputs. To identify the molecular mechanism involved in the sensory experience-induced neural development, we performed a systematic survey of the localization of mRNAs encoding three subtypes of the RGSs (RGS2, RGS4 and RGS7) in developing rat brains by in situ hybridization through postnatal day 2 (P2), P10 and P18 to adult. The most dramatic changes of expression patterns were observed in the discrete neuronal cell layers of the cerebral neocortex (for RGS2 and 4), the hippocampus (for RGS2, 4 and 7), the thalamus (for RGS4) and the cerebellum (for RGS2 and 7). In the neocortex, RGS2 mRNA was enriched in the superficial cortical plate at P2, in contrast to RGS4, which was enriched in more mature neurons of the deeper layer V and VI. In the hippocampus, the neuronal cell layer-specific expression pattern of RGS2 developed from P2 to P18. RGS4 expression was temporarily confined to the CA pyramidal cell layer and not detectable in the dentate gyrus at P10 and P18. Similarly, a high level of expression of RGS7 was observed in the CA area, but not in the dentate gyrus at P2 and P10. In the cerebellum, the maturation of laminar expression patterns for the three RGSs correlated with neuronal maturation and synaptogenesis at P18. The most characteristic temporal pattern among the three RGSs was observed for RGS4 mRNA, which was highly enriched in the thalamocortical regions. The peaks of RGS4 expression were seen in the following regions with distinct onset and duration: the neocortex (from P2 onward), the hippocampus (P10 and P18) and the thalamus (from P18 onward). The divergent temporal and spatial expression of RGS subtypes and their dynamic control in the cortex, the hippocampus and the thalamus suggest that the RGS family could play multiple distinct roles in experience-dependent brain development.
机译：RGS（G蛋白信号调节剂）蛋白在神经元中的大量表达，以及它们对依赖G蛋白的神经传递的调节功能，为细胞适应感觉输入提供了基础。为了确定涉及感觉体验诱导的神经发育的分子机制，我们通过出生后第2天的原位杂交，对发育中的大鼠大脑中编码三种RGS亚型（RGS2，RGS4和RGS7）的mRNA进行了系统的调查。 （P2），P10和P18成人。在大脑新皮层（对于RGS2和4），海马（对于RGS2、4和7），丘脑（对于RGS4）和小脑（对于RGS2和RGS）的离散神经元细胞层中观察到表达模式的最大变化。 7）。在新皮层中，RGS2 mRNA在P2的浅层皮质板中富集，而RGS4在深层V和VI的更成熟的神经元中富集。在海马中，RGS2的神经元细胞层特异性表达模式从P2发展到P18。 RGS4表达暂时局限于CA锥体细胞层，在P10和P18的齿状回中无法检测到。同样，在CA区域观察到RGS7的高水平表达，但在P2和P10的齿状回中却没有。在小脑中，三个RGS的层状表达模式的成熟与P18的神经元成熟和突触发生有关。观察到三个RGS中最具特征的时间模式为RGS4 mRNA，该基因在丘脑皮层区域高度富集。在以下具有不同发作和持续时间的区域中看到了RGS4表达的峰值：新皮层（从P2开始），海马（P10和P18）和丘脑（从P18开始）。 RGS亚型的时空表达及其在皮质，海马和丘脑中的动态控制各不相同，这表明RGS家族可以在依赖经验的大脑发育中发挥多种不同的作用。

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