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首页> 外文期刊>The European Journal of Neuroscience >Swelling-activated calcium signalling in cultured mouse primary sensory neurons.
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Swelling-activated calcium signalling in cultured mouse primary sensory neurons.

机译：培养的小鼠初级感觉神经元中的溶胀激活钙信号传导。

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摘要

The effects of hypo-osmotic membrane stretch on intracellular calcium concentration ([Ca(2+)](i)), cell volume and cellular excitability were investigated in cultured mouse primary sensory trigeminal neurons. Hypotonic solutions (15--45%) led to rapid cell swelling in all neurons. Swelling was accompanied by dose-dependent elevations in [Ca(2+)](i) in a large fraction of neurons. Responses could be classified into three categories. (i) In 57% of the neurons [Ca(2+)](i) responses had a slow rise time and were generally of small amplitude. (ii) In 21% of the neurons, responses had a faster rise and were larger in amplitude. (iii) The remaining cells (22%) did not show [Ca(2+)](i) responses to hypo-osmotic stretch. Slow and fast [Ca(2+)](i) changes were observed in trigeminal neurons of different sizes with variable responses to capsaicin (0.5 microM). The swelling-induced [Ca(2+)](i) responses were not abolished after depletion of intracellular Ca2+ stores with cyclopiazonic acid or preincubation in thapsigargin, but were suppressed in the absence of external Ca(2+). They were strongly attenuated by extracellular nickel and gadolinium. Hypotonic stimulation led to a decrease in input resistance and to membrane potential depolarization. Under voltage-clamp, the [Ca(2+)](i) elevation produced by hypotonic stimulation was accompanied by the development of an inward current and a conductance increase. The time course and amplitude of the [Ca(2+)](i) response to hypo-osmotic stimulation showed a close correlation with electrophysiological properties of the neurons. Fast [Ca(2+)](i) responses were characteristic of trigeminal neurons with short duration action potentials and marked inward rectification. These findings suggest that hypo-osmotic stimulation activates several Ca(2+)-influx pathways, including Gd(3+)-sensitive stretch-activated ion channels, in a large fraction of trigeminal ganglion neurons. Opening of voltage-gated Ca(2+) channels also contributes to the response. The pattern and rate of Ca(2+) influx may be correlated with functional subtypes of sensory neurons.
机译：在培养的小鼠初级感觉三叉神经元中研究了低渗膜拉伸对细胞内钙浓度（[Ca（2 +）]（i）），细胞体积和细胞兴奋性的影响。低渗溶液（15--45％）导致所有神经元细胞迅速肿胀。肿胀伴随着很大一部分神经元中[Ca（2 +）]（i）的剂量依赖性升高。响应可以分为三类。 （i）在57％的神经元中[Ca（2 +）]（i）响应的上升时间较慢，并且幅度通常较小。 （ii）在21％的神经元中，反应的上升较快且振幅较大。 （iii）其余细胞（22％）未显示对低渗拉伸的[Ca（2 +）]（i）反应。在不同大小的三叉神经元中对辣椒素（0.5 microM）具有可变响应，观察到了缓慢和快速的[Ca（2 +）]（i）变化。溶胀诱导的[Ca（2 +）]（i）反应在用环吡嗪酸耗尽细胞内Ca2 +存储或在thapsigargin中预孵育后并未消除，但是在没有外部Ca（2+）时被抑制。它们被细胞外镍和ado强烈衰减。低渗刺激导致输入电阻降低和膜电位去极化。在电压钳位下，由低渗刺激产生的[Ca（2 +）]（i）升高伴随着内向电流的发展和电导的增加。 [Ca（2 +）]（i）对低渗刺激的响应的时间过程和幅度显示与神经元的电生理特性密切相关。快速的[Ca（2 +）]（i）反应是具有短时动作电位和明显向内矫正的三叉神经元的特征。这些发现表明低渗透刺激在大部分三叉神经节神经元中激活了几个Ca（2+）流入途径，包括Gd（3+）敏感的拉伸激活离子通道。电压门控Ca（2+）通道的开放也有助于响应。 Ca（2+）流入的模式和速率可能与感觉神经元的功能亚型相关。

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