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首页> 外文期刊>The European Journal of Neuroscience >Developmental changes in astrocyte density in the macaque perifoveal region.
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Developmental changes in astrocyte density in the macaque perifoveal region.

机译：猕猴中央凹区星形胶质细胞密度的发育变化。

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摘要

We studied astrocyte density both in the perifoveal region and in extrafoveal regions within the same distance of the optic disc (OD) over a time period from foveal pit formation (embryonic day E112) until 2 months after birth. The study was prompted by earlier observations that the adult macaque displays an almost astrocyte-free region around the fovea which, however, at birth is occupied by astrocytes. Thus, we wanted to determine if the perifoveal region is invaded by astrocytes during early development to the same degree as other regions in the central retina, and how the reduction in density can be explained. From the earliest age we studied (embryonic day 112), less astrocytes were found in the perifovea than in other regions equidistant from the OD. In addition, the number of astrocytes steadily declined both in the perifovea and outside until birth. During the first week after birth, there was a further dramatic decline in perifoveal astrocyte density. Double-labelling with glial fibrillary acidic protein (GFAP) immunocytochemistry and the TUNEL method showed that during the whole observation period astrocytes undergo DNA fragmentation and presumably die. However, the rate of TUNEL-positive astrocytes did not significantly differ between perifovea and other regions equidistant to the OD, and at no time did we find a significant peak of apoptosis rate. Thus, the reduction in perifoveal astrocyte density cannot be explained by missing invasion or by selectively elevated apoptosis rates in the foveal and perifoveal regions. Alternative hypotheses are discussed.
机译：我们研究了从小凹坑形成（胚胎日E112）到出生后两个月的一段时间内，在视盘（OD）相同距离内的小凹周围区域和小凹周围区域的星形胶质细胞密度。该研究是由较早的观察结果提示的，成年猕猴在中央凹周围显示出几乎没有星形胶质细胞的区域，但是在出生时却被星形胶质细胞占据。因此，我们想确定在早期发育过程中星形胶质细胞是否侵入了中央凹区，其程度与中央视网膜的其他区域相同，并且如何解释密度的降低。从我们研究的最早年龄（胚胎第112天）开始，在凹凹中发现的星形胶质细胞比在与OD等距的其他区域少。另外，直到产周，在中央凹周围和外部，星形胶质细胞的数目稳定下降。出生后的第一周，小凹周围星形胶质细胞密度进一步急剧下降。用神经胶质纤维酸性蛋白（GFAP）免疫细胞化学和TUNEL方法进行的双重标记显示，在整个观察期内，星形胶质细胞会发生DNA断裂并可能死亡。然而，TUVE阳性星形胶质细胞的比率在凹凹和与OD等距的其他区域之间没有显着差异，并且我们从未发现明显的凋亡率峰值。因此，不能通过缺少侵袭或通过在中央凹和中央凹区域中选择性升高的凋亡率来解释中央凹星形胶质细胞密度的降低。讨论了其他假设。

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