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首页> 外文期刊>The European Journal of Neuroscience >Effect of melatonin administration on qPer2, qPer3, and qClock gene expression in the suprachiasmatic nucleus of Japanese quail.
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Effect of melatonin administration on qPer2, qPer3, and qClock gene expression in the suprachiasmatic nucleus of Japanese quail.

机译：褪黑激素的施用对日本鹌鹑视交叉上核qPer2，qPer3和qClock基因表达的影响。

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摘要

Temporal changes of mRNA expression of three clock genes, qPer2, qPer3 and qClock, were studied in the suprachiasmatic nucleus (SCN) of Japanese quail under different light conditions, as well as under the condition of continuous melatonin. In addition, the expression of melatonin receptor genes, Mel1a and Mel1c, in the SCN were also examined. The expression of qPer2 mRNA showed robust oscillation during both light and dark (LD) 12:12 cycles and under constant dark conditions (DD), but did not exhibit circadian rhythmicity in constant light conditions (LL), instead being expressed at a consistently high level. Expression of qPer3 also showed robust oscillation under both LD and DD conditions. Unlike qPer2 however, qPer3 mRNA expression remained rhythmic under LL conditions. Contrary to the findings on the other clock genes, no remarkable rhythmicity was detectable in either light condition. Both Mel1a and Mel1c mRNAs were detected in the SCN, however, Mel1a mRNA levels were higher than Mel1c and showed daily rhythmicity. Although implantation of melatonin tubes caused constant high levels of plasma melatonin and consequently masked the endogenous daily melatonin rhythm, no significant differences in the expression pattern of any of the three clock genes were observed between birds with and without constant melatonin. In addition, a single injection of melatonin did not affect mRNA expression of these clock genes. These results suggest that melatonin does not affect transcription of clock genes, but may act on the mechanism of synchronization among SCN oscillatory cells.
机译：在不同光照条件下，以及在连续褪黑素条件下，研究了日本鹌鹑的睑上核（SCN）中三个时钟基因qPer2，qPer3和qClock的mRNA表达的时间变化。此外，还检查了褪黑激素受体基因Mel1a和Mel1c在SCN中的表达。 qPer2 mRNA的表达在黑暗和黑暗（LD）12:12周期以及恒定黑暗条件（DD）下均显示出强劲的振荡，但在恒定光照条件（LL）下未显示昼夜节律，而是始终以较高的频率表达水平。 qPer3的表达在LD和DD条件下也显示出稳健的振荡。但是，与qPer2不同，qPer3 mRNA在LL条件下仍保持节律。与其他时钟基因的发现相反，在任何光照条件下均未检测到明显的节律。在SCN中均检测到Mel1a和Mel1c mRNA，但是Mel1a mRNA的水平高于Mel1c，并表现出每日的节律性。尽管植入褪黑素管会导致血浆褪黑素水平持续升高，从而掩盖了每日的内源性褪黑激素节律，但在有和没有恒定褪黑素的鸟类之间，这三个时钟基因的表达模式均未见明显差异。此外，单次注射褪黑激素不会影响这些时钟基因的mRNA表达。这些结果表明褪黑激素不影响时钟基因的转录，但可能作用于SCN振荡细胞之间的同步机制。

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