• 主题
高级搜索  >
历史搜索 清空历史
首页> 外文期刊>The European Journal of Neuroscience >Specific expression of the cell cycle regulation proteins, GADD34 and PCNA, in the peri-infarct zone after focal cerebral ischaemia in the rat.
【24h】

Specific expression of the cell cycle regulation proteins, GADD34 and PCNA, in the peri-infarct zone after focal cerebral ischaemia in the rat.

机译：在大鼠局灶性脑缺血后，梗死周围区域中细胞周期调节蛋白GADD34和PCNA的特异性表达。

获取原文
获取原文并翻译 | 示例
           
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Cell cycle proteins play key roles in cell survival or death under pathological conditions. Expression of growth arrest and DNA damage-inducible protein, GADD34 and proliferating cell nuclear antigen (PCNA) have been investigated in the core and peri-infarct zone at 2 and 24 h after middle cerebral artery occlusion (MCAO). At these times after MCAO, numerous GADD34-positive cells were present, particularly in the peri-infarct zone (e.g. 24 +/- 4 and 52 +/- 6 immunopositive cells/0.25 mm2 at 2 and 24 h, respectively, in cortex). PCNA-immunopositive cells were barely detectable in the peri-infarct zone at 2 h; however, numerous PCNA-immunopositive cells were present in this zone by 24 h (0.7 +/- 0.3 and 10.6 +/- 1.5 immunopositive cells/0.25 mm2, respectively) as well as in the adjacent cortex and in the contralateral cingulate cortex. Most GADD34-immunopositive cells coexpressed the neuronal marker Neu-N with a smaller number coexpressing the microglial marker, Mrf-1. Evidence of morphologically 'abnormal' and 'normal' GADD34 immunopositive neurons was found within the peri-infarct zone. The majority of PCNA immunopositive cells were Mrf-1 positive with a smaller number Neu-N positive. Double-labelling revealed colocalization of GADD34 and PCNA in some cells within the peri-infarct zone and in the ependymal cells lining the ventricles. The presence of GADD34 and PCNA in a key anatomical location pertinent to the evolving ischaemic lesion indicates that GADD34, either alone or in combination with PCNA, has the potential to influence cell survival in ischaemically compromised tissue.
机译：细胞周期蛋白在病理条件下的细胞存活或死亡中起关键作用。已经研究了大脑中动脉闭塞（MCAO）后2小时和24小时，在核心和梗塞周围区域检测了生长停滞和DNA损伤诱导蛋白，GADD34和增殖细胞核抗原（PCNA）的表达。在MCAO后的这些时间，存在大量GADD34阳性细胞，特别是在梗塞周围区域（例如，皮层2和24小时分别为24 +/- 4和52 +/- 6免疫阳性细胞/0.25 mm2）。 。在2 h时，在梗塞周围区域几乎检测不到PCNA免疫阳性细胞。然而，在该区域中，到24小时时，分别存在大量PCNA免疫阳性细胞（分别为0.7 +/- 0.3和10.6 +/- 1.5免疫阳性细胞/0.25 mm2）以及在相邻的皮质和对侧扣带状皮质中。大多数GADD34免疫阳性细胞共表达神经元标记Neu-N，而少部分共表达小胶质标记Mrf-1。在梗死周围区域发现形态学上“异常”和“正常”的GADD34免疫阳性神经元。大多数PCNA免疫阳性细胞是Mrf-1阳性，而少数Neu-N阳性。双重标记揭示了GADD34和PCNA在梗塞周围区域内某些细胞以及在心室内衬的室管膜细胞中的共定位。 GADD34和PCNA在与不断发展的缺血性病变有关的关键解剖位置中的存在表明，GADD34（单独或与PCNA结合使用）有可能影响缺血受损组织中的细胞存活。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
获取原文

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号