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首页> 外文期刊>Neuropathology: official journal of the Japanese Society of Neuropathology >Human embryonic neural stem cell transplantation increases subventricular zone cell proliferation and promotes peri-infarct angiogenesis after focal cerebral ischemia.
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Human embryonic neural stem cell transplantation increases subventricular zone cell proliferation and promotes peri-infarct angiogenesis after focal cerebral ischemia.

机译：人胚胎神经干细胞移植可增加局灶性脑缺血后脑室下区的细胞增殖，并促进梗塞周围的血管生成。

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Neurogenesis and angiogenesis are two important processes that may contribute to the repair of brain injury after stroke. This study was designed to investigate whether transplantation of human embryonic neural stem cells (NSCs) into cortical peri-infarction 24h after ischemia effects cell proliferation in the subventricular zone (SVZ) and angiogenesis in the peri-infarct zone. NSCs were prepared from embryonic human brains at 8 weeks gestation. Focal cerebral ischemia was induced by permanent occlusion of the middle cerebral artery of adult rats. Animals were randomly divided into two groups (n=30, each) at 24h after ischemia: NSC-grafted and medium-grafted groups. Toluidine blue staining and 5'-bromo-2'-deoxyuridine (BrdU) or von Willebrand factor (vWF) immunohistochemistry were performed at 7, 14 and 28 days after transplantation. NSC transplantation increased the number of BrdU-positive cells in the ischemic ipsilateral SVZ compared with the medium control at 7 days (P<0.01). This difference in SVZ cell proliferation persisted at 14 days (P<0.01), but was not significant at 28 days (P>0.05). In addition, angiogenesis, as indicated by BrdU and vWF staining in cortical peri-infarct regions, was augmented by 46% and 65% in NSC-grafted rats versus medium-grafted rats at 7 and 14 days, respectively (P<0.05). However, this increase became non-significant at 28 days (P>0.05). Our results indicate that NSC transplantation enhances endogenous cell proliferation in the SVZ and promotes angiogenesis in the peri-infarct zone, even if it is performed in the acute phase of ischemic injury.
机译：神经发生和血管生成是两个重要过程，可能有助于中风后脑损伤的修复。这项研究旨在调查缺血后24小时是否将人类胚胎神经干细胞（NSCs）移植到皮层梗死区域内，从而影响脑室下区域（SVZ）的细胞增殖和梗塞周围区域的血管生成。在妊娠8周时从胚胎人脑制备NSC。成年大鼠大脑中动脉的永久性闭塞可诱发局灶性脑缺血。在缺血后24小时，将动物随机分为两组（每组n ＝ 30）：NSC移植组和中等移植组。在移植后第7、14和28天进行甲苯胺蓝染色和5'-bromo-2'-脱氧尿苷（BrdU）或von Willebrand因子（vWF）免疫组织化学。与培养基对照组相比，在7天时，NSC移植增加了缺血性同侧SVZ中BrdU阳性细胞的数量（P <0.01）。 SVZ细胞增殖的这种差异在第14天持续存在（P <0.01），而在28天时没有显着差异（P> 0.05）。另外，如在大脑皮层梗塞区域的BrdU和vWF染色所表明的，在NSC移植的大鼠中，在第7天和第14天，血管生成分别增加了46％和65％（P <0.05）。但是，这种增加在28天时变得不显着（P> 0.05）。我们的结果表明，即使在缺血性损伤的急性期进行，NSC移植也能增强SVZ中的内源性细胞增殖并促进梗塞周围区域的血管生成。

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