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首页> 外文期刊>The European Journal of Neuroscience >Cellular architecture of the nucleus reuniens thalami and its putative aspartatergic/glutamatergic projection to the hippocampus and medial septum in the rat.
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Cellular architecture of the nucleus reuniens thalami and its putative aspartatergic/glutamatergic projection to the hippocampus and medial septum in the rat.

机译：大鼠核中丘脑的细胞结构及其推定的天冬氨酸/谷氨酸能投射到大鼠海马和中隔的能力。

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Little is known about the neurochemical features of the nucleus reuniens thalami (RE). In the present study, immunocytochemical experiments were performed to characterize the expression pattern of certain neurochemical markers, e.g. the calcium-binding proteins calbindin and calretinin and several neuropeptides. Colocalization studies revealed that half of the calbindin-positive cells express calretinin, and numerous calretinin-immunoreactive neurons contain calbindin. In contrast, immunolabelling for neuropeptides did not reveal cell bodies in the RE. The RE establishes widespread connections with several limbic structures. To correlate these projection patterns with the neurochemical characteristics of RE neurons, the retrograde tracer [3H]d-aspartate, which is selectively taken up by high affinity uptake sites that use glutamate as neurotransmitter, and the nonselective retrograde tracer wheatgerm agglutinin-conjugated colloidal gold was injected into the stratum lacunosum moleculare of the hippocampal CA1 subfield and into the medial septum. The results provide direct anatomical demonstration of aspartatergic/glutamatergic projection from the RE to the hippocampus and to the medial septum. Nearly all of the projecting neurons proved to be calbindin-immunopositive and many of them expressed calretinin. Both retrograde labelling techniques revealed that neurons projecting to the hippocampus were located in clusters in the dorsolateral part of the RE, whereas neurons projecting to the medial septum were mainly distributed in the ventromedial portion of the nucleus, indicating that different cell populations project to these limbic areas. These results suggest that neurons in the RE are heterogeneous and contribute to the excitatory innervation of the septo-hippocampal system.
机译：鲜有关于瑞尼氏核（RE）的神经化学特征的了解。在本研究中，进行了免疫细胞化学实验来表征某些神经化学标记物，例如神经营养标记物的表达模式。钙结合蛋白calbindin和calretinin以及一些神经肽。共定位研究表明，calbindin阳性细胞中有一半表达calretinin，许多calretinin免疫反应性神经元都含有calbindin。相反，对神经肽进行免疫标记并没有揭示RE中的细胞体。 RE建立了与几个边缘结构的广泛连接。为了将这些投影模式与RE神经元的神经化学特征相关联，使用谷氨酸作为神经递质的高亲和力摄取位点选择性摄取逆行示踪剂[3H] d-天冬氨酸，以及非选择性逆行示踪剂小麦胚芽凝集素缀合的胶体金将其注射到海马CA1子区的腔层分子中和内侧隔中。结果提供了从RE到海马和内侧中隔的天冬氨酸/谷氨酸能投射的直接解剖学证明。几乎所有的投射神经元都被证明是钙结合蛋白免疫阳性的，其中许多都表达钙调蛋白。两种逆行标记技术都表明，投射到海马的神经元位于RE背外侧部分的簇中，而投射到中隔的神经元主要分布在核的腹膜部分，表明不同的细胞群投射到这些边缘地区。这些结果表明，RE中的神经元是异质的，并有助于隔垫海马系统的兴奋性神经支配。

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