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首页> 外文期刊>The European Journal of Neuroscience >In vivo regulation of dopamine and noradrenaline release by alpha2A-adrenoceptors in the mouse prefrontal cortex.
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In vivo regulation of dopamine and noradrenaline release by alpha2A-adrenoceptors in the mouse prefrontal cortex.

机译：小鼠前额叶皮层中α2A-肾上腺素受体对多巴胺和去甲肾上腺素释放的体内调节。

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The present study investigated the role of alpha2A-adrenoceptor subtype in the regulation of noradrenaline and dopamine release in the medial prefrontal cortex. The effect of local introduction of the alpha2-adrenoceptor agonist dexmedetomidine (10-9-10-8 m) on noradrenaline and dopamine release was investigated in alpha2A-adrenoceptor knockout and control mice by using in vivo microdialysis. Furthermore, to reveal a possible distinction between regulation of baseline and peak release, we sampled the dialysate during both rest and handling-induced mild stress. Baseline noradrenaline and dopamine concentrations did not differ between alpha2A-adrenoceptor knockout and control mice. Dexmedetomidine decreased, in a concentration-dependent manner, noradrenaline and dopamine levels in both genotypes. However, the effect of dexmedetomidine on noradrenaline release was attenuated in the alpha2A-adrenoceptor knockout mice, whereas the effect on dopamine release did not differ between the genotypes. The first handling episode increased noradrenaline and dopamine levels to the same extent in both genotypes. However, in alpha2A-adrenoceptor knockout mice the noradrenaline and dopamine levels remained elevated in the samples following the first handling whilst, in the control mice, transmitter levels returned to baseline levels. In control mice the handling-induced peak noradrenaline and dopamine levels were lower after the administration of dexmedetomidine than during the first handling episode, but in alpha2A-adrenoceptor knockout mice no drug effect on handling-induced peak noradrenaline and dopamine levels was found. Our results suggest that the release of noradrenaline in the medial prefrontal cortex is mainly regulated via alpha2A-adrenoceptors, whilst other alpha-adrenoceptor subtypes play a significant role in the regulation of dopamine release.
机译：本研究调查了α2A-肾上腺素受体亚型在去甲肾上腺素和多巴胺释放在前额叶内侧皮质中的调节作用。通过使用体内微透析研究了α2A-肾上腺素受体敲除和对照小鼠中局部引入α2-肾上腺素受体激动剂右美托咪定（10-9-10-8 m）对去甲肾上腺素和多巴胺释放的影响。此外，为了揭示基线调节和峰值释放之间的可能区别，我们在休息和处理引起的轻度压力下对透析液进行了采样。基线去甲肾上腺素和多巴胺浓度在α2A-肾上腺素受体基因敲除小鼠和对照组小鼠之间没有差异。在两种基因型中，右美托咪定以浓度依赖性方式降低去甲肾上腺素和多巴胺水平。但是，右旋美托咪定对去甲肾上腺素释放的影响在α2A-肾上腺素受体敲除小鼠中减弱了，而多巴胺释放的影响在基因型之间没有差异。在两种基因型中，第一次处理发作均使去甲肾上腺素和多巴胺水平升高至相同程度。但是，在α2A-肾上腺素受体剔除小鼠中，首次处理后，样品中的去甲肾上腺素和多巴胺水平仍然升高，而在对照小鼠中，递质水平恢复到基线水平。在对照组小鼠中，右美托咪定给药后诱发的去甲肾上腺素和多巴胺的峰值水平低于第一次处理发作期间，但在alpha2A-肾上腺素受体敲除小鼠中未发现对诱发诱发的去甲肾上腺素和多巴胺水平产生药物作用。我们的结果表明，去甲肾上腺素在内侧前额叶皮层中的释放主要通过α2A-肾上腺素受体来调节，而其他α-肾上腺素受体亚型在多巴胺释放的调节中起重要作用。

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