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首页> 外文期刊>The European Journal of Neuroscience >Relationship between two types of vesicular glutamate transporters and neurokinin-1 receptor-immunoreactive neurons in the pre-Botzinger complex of rats: light and electron microscopic studies.
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Relationship between two types of vesicular glutamate transporters and neurokinin-1 receptor-immunoreactive neurons in the pre-Botzinger complex of rats: light and electron microscopic studies.

机译：博兹格前脑复合体中两种类型的囊泡谷氨酸转运蛋白与神经激肽-1受体免疫反应性神经元之间的关系：光镜和电镜观察。

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摘要

Our previous study demonstrated GABAergic and glycinergic synapses onto neurokinin-1 receptor (NK1R)-immunoreactive (ir) neurons in the pre-Botzinger complex (pre-BotC), the hypothesized kernel of normal respiratory rhythmogenesis. In the present study, we aimed to identify glutamatergic synapses onto NK1R-ir pre-BotC neurons, as excitatory synaptic transmission is a prerequisite to normal respiratory rhythmogenesis. Two types of vesicular glutamate transporters (VGLUT), VGLUT1 and VGLUT2, have been recently implicated in glutamate-mediated transmission. The present study used immunofluorescence and immunogold-silver staining to determine the relationship between the transporters and NK1R-ir neurons in the pre-BotC of adult rats. Under the confocal laser-scanning microscope, VGLUT2-ir boutons were found to be widely distributed in the pre-BotC, some of which were in close apposition to NK1R-ir somas and dendrites. VGLUT1-ir boutons were relatively rare and only a few were found to be in close apposition to NK1R-ir somas and dendrites. Electron microscopic observation revealed that approximately 41% of VGLUT2-ir terminals were in close apposition to, or made asymmetric synapses with NK1R-ir somas and dendrites in the pre-BotC. On the other hand, 50.5% of NK1R-ir dendrites were closely apposed to, or synapsed with VGLUT2-ir terminals. Occasionally, VGLUT1-ir terminals were found in close apposition to NK1R-ir somas or dendrites, but we were unable to identify synapses between them. The present findings provide the morphological basis for excitatory synaptic inputs onto NK1R-ir neurons in the pre-BotC. VGLUT2 may be involved in a dominant excitatory synaptic pathway for normal respiratory rhythmogenesis.
机译：我们以前的研究表明，在正常的呼吸节律发生的假想核心波兹辛前体（pre-BotC）中，神经氨酸1受体（NK1R）-免疫反应性（ir）神经元上有GABA能和甘氨酸能突触。在本研究中，我们旨在确定NK1R-ir前BotC神经元上的谷氨酸能突触，因为兴奋性突触传递是正常呼吸节律发生的先决条件。最近已经涉及到两种类型的囊泡谷氨酸转运蛋白（VGLUT）和VGLUT1和VGLUT2与谷氨酸介导的传递有关。本研究使用免疫荧光和免疫金银染色确定成年大鼠pre-BotC中转运蛋白与NK1R-ir神经元之间的关系。在共聚焦激光扫描显微镜下，发现VGLUT2-ir布顿广泛分布在pre-BotC中，其中一些与NK1R-ir体和树突紧邻。 VGLUT1-ir boutons相对较少，仅发现少数与NK1R-ir体和树突并列。电子显微镜观察显示，大约41％的VGLUT2-ir末端与pre-BotC中的NK1R-ir体和树突紧密贴合，或与它们不对称突触。另一方面，NK1R-ir树突的50.5％与VGLUT2-ir末端紧密结合或突触。有时，发现VGLUT1-ir末端与NK1R-ir体或树突紧密相邻，但我们无法识别它们之间的突触。目前的发现为BotC前的NK1R-ir神经元兴奋性突触输入提供了形态学基础。 VGLUT2可能参与正常呼吸节律发生的主要兴奋性突触途径。

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