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首页> 外文期刊>The European Journal of Neuroscience >Neurons of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue express the prostaglandin E receptor subtype EP3.
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Neurons of the rat preoptic area and the raphe pallidus nucleus innervating the brown adipose tissue express the prostaglandin E receptor subtype EP3.

机译：大鼠视前区的神经元和神经支配棕色脂肪组织的睑裂神经核表达前列腺素E受体亚型EP3。

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摘要

The major effector organ for thermogenesis during inflammation or experimental pyrogen-induced fever in rodents is the brown adipose tissue (BAT). Prostaglandin E2 (PGE2) microinjection into the medial preoptic area (POA) of rats leads to hyperthermia through an increase in BAT thermogenesis and induces pyrogenic signal transmission towards the raphe pallidus nucleus (RPa), a brainstem nucleus known to contain sympathetic premotor neurons for BAT control. The medial POA has a high expression of prostaglandin E receptor subtype EP3 (EP3R) on POA neurons, suggesting that these EP3R are main central targets of PGE2 to mediate BAT thermogenesis. To reveal central command neurons that contain EP3R and polysynaptically project to the BAT, we combined EP3R immunohistochemistry with the detection of transneuronally labelled neurons that were infected after injection of pseudorabies virus into the BAT. Neurons double-labelled with EP3R and viral surface antigens were particularly numerous in two brain regions, the medial POA and the RPa. Of all medial POA neurons that became virally infected 71 h after BAT inoculation, about 40% expressed the EP3R. This subpopulation of POA neurons is the origin of a complete neuronal chain that connects potential PGE2-sensitive POA neurons with the BAT. As for the efferent pathway of pyrogenic signal transmission, we hypothesize that neurons of this subpopulation of EP3R expressing POA neurons convey their pyrogenic signals towards the BAT via the RPa. We additionally observed that two-thirds of those RPa neurons that polysynaptically project to the interscapular BAT also expressed the EP3R, suggesting that RPa neurons themselves might possess prostaglandin sensitivity that is able to modulate BAT thermogenesis under febrile conditions.
机译：啮齿动物在炎症或实验性热原诱导的发烧过程中生热的主要效应器官是棕色脂肪组织（BAT）。将大鼠前列腺素E2（PGE2）微注射到大鼠的内侧视前区（POA）中，通过增加BAT生热作用导致体温过高，并导致热原信号传递到麻痹苍白核（RPa），该脑干核是已知含有BAT交感神经运动前神经元的脑干核控制。内侧POA在POA神经元上高表达前列腺素E受体亚型EP3（EP3R），表明这些EP3R是PGE2介导BAT生热的主要中心靶标。为了揭示包含EP3R并多突触地投射到BAT的中枢命令神经元，我们将EP3R免疫组织化学与检测伪狂犬病病毒注入BAT后被感染的经跨神经元标记的神经元相结合。用EP3R和病毒表面抗原双重标记的神经元在两个大脑区域（内侧POA和RPa）特别多。在BAT接种后71小时被病毒感染的所有内侧POA神经元中，约40％表达了EP3R。 POA神经元的这种亚群是连接潜在的PGE2敏感的POA神经元和BAT的完整神经元链的起源。至于热原性信号传递的传出途径，我们假设表达EP3R的POA神经元的这一亚群的神经元通过RPa向BAT传递其热原性信号。我们还观察到，多突触地投射到肩inter间BAT的RPa神经元中有三分之二也表达了EP3R，这表明RPa神经元本身可能具有前列腺素敏感性，能够调节高热条件下BAT生热。

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