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首页> 外文期刊>The European Journal of Neuroscience >Disabled-1 mRNA and protein expression in developing human cortex.
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Disabled-1 mRNA and protein expression in developing human cortex.

机译：发育中的人类皮层中的Disabled-1 mRNA和蛋白质表达。

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摘要

Disabled-1 (Dab1) forms part of the Reelin-Dab1 signalling pathway that controls neuronal positioning during brain development; Dab1 deficiency gives rise to a reeler-like inversion of cortical layers. To establish a timetable of Dab1 expression in developing human brain, Dab1 mRNA and protein expression were studied in prenatal human cortex. The earliest Dab1 signal was detected at 7 gestational weeks (GW), the stage of transition from preplate to cortical plate, suggesting a role of the Reelin-Dab1 signalling pathway in preplate partition. From 12 to 20 GW, the period of maximum cortical migration, Dab1 expression was prominent in the upper tiers of the cortical plate, to decline after midgestation. Radially orientated apical dendrites of Dab1-expressing neurons indicated a predominant pyramidal phenotype. Pyramidal cells in hippocampus and entorhinal cortex displayed a more protracted time of Dab1 expression compared to neocortex. In addition, at later stages (18-25 GW), Dab1 was also expressed in large neurons scattered throughout intermediate zone and subplate. From 14 to 22 GW, particularly high levels of Dab1 mRNA and protein were observed in cells of the ventricular/subventricular zone displaying the morphology of radial glia. The partial colocalization of vimentin and Dab1 in cells of the ventricular zone supported a radial glia phenotype. The concentration of Dab1 protein in ventricular endfeet and initial portions of radial processes of ventricular-zone cells points to a possible involvement of Dab1 in neurogenesis. Furthermore, a subset of Cajal-Retzius cells in the marginal zone colocalized Dab1 and Reelin, and may thus represent a novel target of the Reelin-Dab1 signalling pathway.
机译：Disabled-1（Dab1）是Reelin-Dab1信号通路的一部分，该通路控制大脑发育过程中的神经元定位。 Dab1缺乏症会导致类似reeler的皮质层倒置。为了建立在发育中的人脑中Dab1表达的时间表，在产前人皮质中研究了Dab1 mRNA和蛋白表达。最早的Dab1信号是在妊娠7周（从预板过渡到皮质板的阶段）被检测到的，表明Reelin-Dab1信号通路在预板分配中的作用。从12到20 GW（最大的皮层迁移期），Dab1表达在皮层板的上层突出，在妊娠中期后下降。表达Dab1的神经元的放射状的顶端树突表明主要的锥体表型。与新皮层相比，海马和内嗅皮层中的锥体细胞显示了更长的Dab1表达时间。此外，在后期（18-25 GW），Dab1还表达在散布在整个中间区和亚板中的大神经元中。从14到22 GW，在心室/心室下区的细胞中观察到尤其高水平的Dab1 mRNA和蛋白质，显示了放射状胶质细胞的形态。波形蛋白和Dab1在心室区细胞中的部分共定位支持放射状胶质细胞表型。心室末端和心室区细胞radial突开始部分中Dab1蛋白的浓度表明Dab1可能参与神经发生。此外，边缘区域中的Cajal-Retzius细胞子集共定位了Dab1和Reelin，因此可能代表Reelin-Dab1信号通路的新靶标。

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