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首页> 外文期刊>The European Journal of Neuroscience >Perturbation of CD44 function affects chiasmatic routing of retinal axons in brain slice preparations of the mouse retinofugal pathway.
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Perturbation of CD44 function affects chiasmatic routing of retinal axons in brain slice preparations of the mouse retinofugal pathway.

机译：CD44功能的扰动会影响小鼠视网膜视网膜真菌途径的脑切片制剂中视网膜轴突的嵌合路径。

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摘要

Neurons generated early in development of the ventral diencephalon have been shown to play a key role in defining the midline and the caudal boundary of the optic chiasm in the mouse retinofugal pathway. These functions have been attributed to a surface bound adhesion molecule, CD44 that is expressed in these chiasmatic neurons. In this study, we investigated the effects of perturbing normal CD44 functions on axon routing in brain slice preparations of the mouse retinofugal pathway. Two CD44 antibodies (Hermes-1 and IM7) were used that bind to distinct epitopes on the extracellular domain of the molecule. We found that both antibodies produced dramatic defects in routing of the retinal axons that arrive early in the chiasm. In preparations of embryonic day 13 (E13) and E14 pathways, the crossed component in the chiasm was significantly reduced after antibody treatment. However, such reduction in axon crossing was not observed in E15 chiasm, indicating that the lately generated crossed axons lost their responses to CD44. Furthermore, the anti-CD44 treatment produced a reduction in the uncrossed component in the E15 but not in younger pathways, suggesting a selective response of the lately generated axons, mostly from ventral temporal retina, but not those generated earlier, to the CD44 at the chiasmatic midline in order to make their turn for the uncrossed pathway. These findings provide evidence that a normal function of CD44 molecules in the chiasmatic neurons is essential for axon crossing and axon divergence at the mouse optic chiasm.
机译：已显示在腹侧间脑的早期发育中产生的神经元在定义小鼠视网膜黄体途径中视交叉的中线和尾端边界中起关键作用。这些功能归因于表面结合的粘附分子CD44，这些分子在这些chi神经元中表达。在这项研究中，我们调查了正常的CD44功能对小鼠视网膜视网膜真菌途径脑切片制剂中轴突路由的影响。使用了两种CD44抗体（Hermes-1和IM7），它们与分子胞外域上的不同表位结合。我们发现这两种抗体在早期到达视网膜的视网膜轴突的路由中产生了严重的缺陷。在胚胎第13天（E13）和E14途径的制备中，经过抗体处理后，大交叉中的交叉成分显着减少。但是，这种轴突交叉减少没有在E15交叉症中观察到，表明最近产生的交叉轴突失去了对CD44的反应。此外，抗CD44处理可减少E15中未交联的成分，但不会减少年轻的途径，这表明后期产生的轴突（主要来自腹侧视网膜，而非较早产生的轴突）对CD44处的选择性反应。正中线以使他们转向未跨越的道路。这些发现提供了证据，表明嵌合神经元中CD44分子的正常功能对于小鼠视神经交叉处的轴突交叉和轴突发散至关重要。

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