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首页> 外文期刊>The European Journal of Neuroscience >Distribution of HCN1 and HCN2 in rat auditory brainstem nuclei.
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Distribution of HCN1 and HCN2 in rat auditory brainstem nuclei.

机译：HCN1和HCN2在大鼠听性脑干核中的分布。

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Abstract Auditory brainstem neurons that are involved in the precise analysis of the temporal pattern of sounds have ionic currents activated near the resting potential to shorten membrane time constants. One of these currents is the hyperpolarization-activated current (Ih). Molecular cloning of the channels underlying Ih revealed four different isoforms (HCN1-4). HCN1 and HCN2, which are widely distributed in the brain, differ in their activation kinetics, voltage dependence and sensitivity to cAMP. We determined the distribution of the HCN1 and HCN2 isoform in the auditory brainstem and midbrain of young rats (P20-30), using standard immunohistochemical techniques. HCN1 antibodies gave rise to punctate staining on the somatic and dendritic membrane. Strong HCN1 staining was present on octopus and bushy cells of the ventral cochlear nucleus, principal neurons of the lateral and medial superior olive, and neurons of the ventral nucleus of the lateral lemniscus. No HCN1 staining was observed in the dorsal cochlear nucleus and the medial nucleus of the trapezoid body (MNTB). In contrast, HCN2 staining was strongest in the MNTB and the dorsal nucleus of the lateral lemniscus. Strong HCN2 antibody labelling was also observed in bushy cells of the ventral cochlear nucleus. In the central nucleus of the inferior colliculus only a subpopulation of neurons showed HCN1 or HCN2 immunolabelling. This differential distribution of HCN1 and HCN2 channels is in agreement with the physiologically observed Ih currents in corresponding neuronal populations and might represent the basis for functional heterogeneity and diverse sensitivity to neuromodulators.
机译：摘要参与声音时间模式精确分析的听觉脑干神经元在静息电位附近激活了离子电流，从而缩短了膜时间常数。这些电流之一是超极化激活电流（Ih）。 Ih潜在通道的分子克隆揭示了四种不同的同工型（HCN1-4）。 HCN1和HCN2广泛分布在大脑中，其激活动力学，电压依赖性和对cAMP的敏感性不同。我们使用标准的免疫组织化学技术确定了幼鼠（P20-30）的听觉脑干和中脑中HCN1和HCN2亚型的分布。 HCN1抗体在体细胞和树突状膜上产生了点状染色。 HCN1的强染色存在于腹侧耳蜗核的章鱼和丛状细胞，外侧和内侧上橄榄的主要神经元以及外侧马鞭草的腹核的神经元上。在梯形体（MNTB）的背侧耳蜗核和内侧核中未观察到HCN1染色。相比之下，HCN2染色在MNTB和侧生双侧莲核的背核中最强。在腹侧耳蜗核的浓密细胞中也观察到强HCN2抗体标记。在下丘脑中央核中，只有一部分神经元显示出HCN1或HCN2免疫标记。 HCN1和HCN2通道的这种差异分布与相应神经元群体中生理观察到的Ih电流一致，并且可能代表了功能异质性和对神经调节剂的多种敏感性的基础。

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