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首页> 外文期刊>The European Journal of Neuroscience >GABAergic neurons participate in the brain's response to birdsong auditory stimulation.
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GABAergic neurons participate in the brain's response to birdsong auditory stimulation.

机译：GABA能神经元参与大脑对鸟鸣听觉刺激的反应。

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Abstract Birdsong is a learned vocal behaviour that requires intact hearing for its development in juveniles and for its maintenance during adulthood. However, the functional organization of the brain circuits involved in the perceptual processing of song has remained obscure. Here we provide evidence that GABAergic mechanisms are an important component of these circuits and participate in the auditory processing of birdsong. We first cloned a zebra finch homologue of the gene encoding the 65-kDa isoform of glutamic acid decarboxylase (zGAD-65), a specific GABAergic marker, and conducted an expression analysis by in situ hybridization to identify GABAergic cells and to map their distribution throughout auditory telencephalic areas. The results showed that field L2, the caudomedial nidopallium (NCM) and the caudomedial mesopallium (CMM) contain a high number of GABAergic cells. Using patch-clamp brain slice recordings, we found abundant GABAergic mIPSCs in NCM. Pharmacological antagonism of mIPSCs induced large EPSC bursts, suggesting that tonic inhibition helps to stabilize NCM against runaway excitation via activation of GABA-A receptors. Next, using double fluorescence in situ hybridization and double immunocytochemical labelling, we demonstrated that large numbers of GABAergic cells in NCM and CMM show inducible expression of the transcriptional regulator ZENK in response to song auditory stimulation. These data provide direct evidence that GABAergic neurons in auditory brain regions are activated by song stimulation. Altogether, our results suggest that GABAergic mechanisms participate in auditory processing and perception, and might contribute to the memorization of birdsong.
机译：摘要鸟鸣是一种博学的声音行为，需要完整的听觉才能使其在青少年中发育并在成年期间得到维持。然而，涉及歌曲的感知处理的脑电路的功能组织仍然不清楚。在这里，我们提供的证据表明，GABA能机制是这些回路的重要组成部分，并参与了鸟鸣的听觉处理。我们首先克隆了编码GABA特异标记的谷氨酸脱羧酶65 kDa同工型（zGAD-65）的基因的斑马雀科同源物，并通过原位杂交进行了表达分析，以鉴定GABA能细胞并绘制其分布图。听觉脑脑区。结果表明，场L2，伪内侧膜神经元（NCM）和伪内侧膜神经元（CMM）包含大量的GABA能细胞。使用膜片钳脑切片记录，我们在NCM中发现了丰富的GABA能mIPSC。 mIPSCs的药理拮抗作用可引起较大的EPSC爆发，这表明补品抑制作用可通过激活GABA-A受体来帮助稳定NCM抵抗失控的兴奋。接下来，使用双重荧光原位杂交和双重免疫细胞化学标记，我们证明了NCM和CMM中的大量GABA能细胞表现出可诱导表达的转录调节子ZENK响应于听觉听觉刺激。这些数据提供直接的证据表明，听觉脑区域中的GABA能神经元被歌曲刺激激活。总之，我们的结果表明，GABA能机制参与听觉处理和知觉，并且可能有助于记忆鸟鸣。

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