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首页> 外文期刊>The European Journal of Neuroscience >Nxf and Fbxo33: novel seizure-responsive genes in mice.
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Nxf and Fbxo33: novel seizure-responsive genes in mice.

机译：Nxf和Fbxo33：小鼠中新型的癫痫发作反应基因。

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Abstract Much is understood about the response of the brain to seizure but little is known in relation to the underlying molecular mechanisms involved. We used microarray technology to investigate the complex genetic response of the brain to generalized seizure. For this investigation a seizure-specific mouse brain cDNA library was generated and spotted onto microarray slides with the aim of increasing the likelihood of identifying novel genes responsive to seizure. Microarray analysis was performed on mouse hippocampus 1 h after generalized seizure pharmacologically induced by pentylenetetrazol (PTZ). Using the custom microarray slides, six genes were identified as being up-regulated in this seizure model and results were validated by real-time PCR. Four of the seizure-responsive genes had previously-reported roles in apoptosis, proliferation or differentiation of neural cells. Two of the genes were novel and in situ hybridization analysis demonstrated heightened mRNA expression in the hippocampus 1 hfollowing generalized convulsive seizure, in a pattern which is typical for other activity-dependant genes expressed in this structure. In addition to being up-regulated postseizure, the genes described in this paper appear to be expressed normally in the adult hippocampus and during development.
机译：摘要人们对大脑对癫痫的反应了解很多，但对涉及的潜在分子机制知之甚少。我们使用微阵列技术研究了大脑对全身性癫痫发作的复杂遗传反应。为了进行这项研究，生成了癫痫发作特异性的小鼠大脑cDNA文库，并将其点样到微阵列载玻片上，目的是增加识别对癫痫发作有反应的新基因的可能性。戊四氮唑（PTZ）药理诱导的全身性癫痫发作后1 h，对小鼠海马进行芯片分析。使用定制的微阵列玻片，在该癫痫发作模型中鉴定出六个基因上调，并通过实时PCR验证了结果。癫痫发作应答基因中有四个在神经细胞的凋亡，增殖或分化中具有先前报道的作用。其中两个基因是新颖的，并且原位杂交分析表明，在全身性惊厥发作后，海马中的mRNA表达升高，这种模式是这种结构中表达的其他活性依赖基因的典型特征。除了癫痫发作后上调外，本文描述的基因似乎在成年海马和发育过程中正常表达。

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