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首页> 外文期刊>The European Journal of Neuroscience >Anatomical and functional evidence for a role of arginine-vasopressin (AVP) in rat olfactory epithelium cells.
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Anatomical and functional evidence for a role of arginine-vasopressin (AVP) in rat olfactory epithelium cells.

机译：精氨酸加压素（AVP）在大鼠嗅觉上皮细胞中的作用的解剖和功能证据。

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Abstract The olfactory epithelium (OE) is composed of olfactory sensory neurons (OSNs) and sustentacular cells; it lies in the nasal cavity where it is protected by a thin mucus layer. The finely regulated composition of this mucus provides OSN with a suitable ionic environment. To maintain the functional integrity of the epithelium despite permanent physical, chemical and microbial aggressions, both OSNs and surrounding sustentacular cells are continuously renewed from globose basal cells. Moreover, the sense of smell is involved in so numerous behaviours (feeding, reproduction, etc.) that it has to cross-talk with the endocrine and neuroendocrine systems. Thus, besides its sensory function, the olfactory epithelium is thought to undergo a lot of complex regulatory processes. We therefore studied the effects of various neuropeptides on primary cultures of Sprague-Dawley rat olfactory epithelium cells. We found that arginine-vasopressin (AVP) triggered a robust, dose-dependent calcium increase in thesecells. The cell response was essentially ascribed to the V1a AVP receptor, whose presence was confirmed by RT-PCR and immunolabelling. In the culture, V1a but not V1b receptors were present, mainly localized in neurons. In the epithelium, both subtypes were found differentially distributed. V1a-R were localized mainly in globose basal cells and at the apical side of the epithelium, in the area of the dendritic knobs of OSNs. V1b-R were strongly associated with Bowman's gland cells and globose basal cells. These localizations suggested potential multifaceted roles of a hormone, AVP, in the olfactory epithelium.
机译：摘要嗅觉上皮（OE）由嗅觉感觉神经元（OSNs）和鳞状上皮细胞组成。它位于鼻腔中，并由一薄层粘液层保护。该粘液的精细调节成分为OSN提供了合适的离子环境。尽管存在永久性的物理，化学和微生物侵袭，但为了维持上皮的功能完整性，OSN和周围的Sustentacular细胞都不断从球状基底细胞更新。此外，气味感涉及许多行为（进食，繁殖等），以至于它必须与内分泌和神经内分泌系统发生串扰。因此，除了其感觉功能外，嗅觉上皮还被认为经历了许多复杂的调节过程。因此，我们研究了各种神经肽对Sprague-Dawley大鼠嗅上皮细胞原代培养的影响。我们发现精氨酸加压素（AVP）触发了这些细胞中强大的，剂量依赖性的钙增加。细胞反应本质上归因于V1a AVP受体，通过RT-PCR和免疫标记证实了其存在。在培养物中，存在V1a受体但不存在V1b受体，主要位于神经元中。在上皮中，发现两种亚型差异分布。 V1a-R主要位于OSNs的树突状突起区域的球状基底细胞和上皮的顶侧。 V1b-R与鲍曼氏腺细胞和球状基底细胞密切相关。这些定位表明，激素AVP在嗅觉上皮细胞中可能具有多种作用。

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