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首页> 外文期刊>The European Journal of Neuroscience >Modulation of feeding behaviour by blocking purinergic receptors in the rat nucleus accumbens: a combined microdialysis, electroencephalographic and behavioural study.
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Modulation of feeding behaviour by blocking purinergic receptors in the rat nucleus accumbens: a combined microdialysis, electroencephalographic and behavioural study.

机译：通过阻断大鼠伏隔核中嘌呤能受体来调节喂养行为：微透析，脑电图和行为研究的组合。

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The nonspecific P2 receptor antagonist pyridoxalphosphate-6-azophenyl-2',4'-disulphonic acid (PPADS), the nonspecific P1 receptor antagonist 8-(p-sulphophenyl)-theophylline (8-SPT) and the combination of both were applied by retrograde microdialysis into the nucleus accumbens (NAc) before and during feeding of 18-h food-deprived rats. In addition to the registration of behavioural parameters, such as the amount and duration of food intake, the feeding-induced changes in dopamine (DA) concentration and the concomitant changes of neuronal activity in the NAc and the ventral tegmental area (VTA) were simultaneously determined. The perfusion with PPADS (20 microm) diminished the amount of food intake and the duration of feeding. Furthermore, the P2 receptor antagonist blocked the feeding-induced DA release and prevented the feeding-elicited changes of the electroencephalography (EEG) power distribution which was characterised by an increase in the power of the 8.0-13.0-Hz frequency band in the NAc and the VTA. The effects of PPADS could be completely prevented by the concomitantly perfused adenosine receptor antagonist 8-SPT (100 microm). When given alone, 8-SPT increased the amount of food ingested, the duration of feeding and the EEG power of the higher frequency range, particularly between 19.0 and 30.0 Hz, in both the NAc and the VTA. The feeding-elicited DA release was supplemented to the enhanced DA level caused by the perfusion with 8-SPT in an additive manner. The P2 and P1 receptor antagonists interact antagonistically in the modulation of feeding behaviour and the feeding-induced changes of EEG activity suggesting that both endogenous extracellular ATP and adenosine are involved in the regulation of the feeding-associated mesolimbic neuronal activity in a functionally antagonistic manner.
机译：非特异性P2受体拮抗剂吡ido草磷酸-6-偶氮苯基-2'，4'-二磺酸（PPADS），非特异性P1受体拮抗剂8-（对-磺苯基）-茶碱（8-SPT）以及两者的结合使用在进食18小时食物匮乏的大鼠之前和期间，将微量透析逆行进行到伏隔核（NAc）中。除了行为参数的记录（例如食物摄入量和持续时间）外，喂养引起的多巴胺（DA）浓度变化以及NAc和腹侧被盖区（VTA）中神经元活动的伴随变化也同时发生决心。 PPADS（20微米）的灌注减少了食物摄入量和喂养时间。此外，P2受体拮抗剂阻断了进食引起的DA释放，并阻止了进食引起的脑电图（EEG）功率分布的变化，其特征是NAc和8.0-13.0-Hz频段功率的增加。 VTA。伴随灌注的腺苷受体拮抗剂8-SPT（100 microm）可以完全防止PPADS的作用。如果单独使用8-SPT，则NAc和VTA均会增加摄食量，进食时间和较高频率范围（尤其在19.0至30.0 Hz之间）的EEG功率。饲喂引起的DA释放以添加方式补充了由8-SPT灌注引起的DA水平升高。 P2和P1受体拮抗剂在调节进食行为和进食诱导的EEG活性变化中发生拮抗作用，表明内源性细胞外ATP和腺苷均以功能拮抗的方式参与与进食相关的中脑边缘神经元活性的调节。

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