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首页> 外文期刊>The European Journal of Neuroscience >Clock gene mRNA and protein rhythms in the pineal gland of mice.
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Clock gene mRNA and protein rhythms in the pineal gland of mice.

机译：小鼠松果体中的Clock基因mRNA和蛋白节律。

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In vertebrates, the rhythmic transcription of clock genes, regulated by their own gene products, provides the basis for self-sustaining circadian clockworks. These endogenous clocks are lost in most mammalian tissues, but not in the central pacemaker of the hypothalamic suprachiasmatic nucleus (SCN). An interesting model system to understand this phylogenetic shift in function of clock gene products is the rodent pineal gland, as its intrinsic clockwork was replaced during evolution by an input-dependent oscillator. By means of immunohistochemistry, immunoblotting and real time PCR, we investigated the dayight expression profiles of all major clock genes and their products in the pineal gland of one melatonin-proficient and one melatonin-deficient mouse strain. All clockwork elements known to be indispensable for a sustained rhythm generation in the SCN were also found in the pineal organ of both mouse strains. Only mPer1 mRNA and PER1 protein accumulation coincides with timecourses of many other pineal genes and their products, which are cyclicAMP inducible. Here, presented data together with the known mechanisms for regulation of the mPer1 gene in the rodent pineal gland forward the idea that in this tissue PER1 may have a trigger function for initiating the cycles of the clockwork's transcriptional/translational feedback loops.
机译：在脊椎动物中，时钟基因的节律性转录受其自身的基因产物调节，为自我维持的生物钟提供了基础。这些内源时钟在大多数哺乳动物组织中丢失，但在下丘脑上视交叉上核（SCN）的中央起搏器中未丢失。啮齿类动物的松果体是了解时钟基因产物功能的这种系统发生变化的有趣模型系统，因为其内在的发条在进化过程中被依赖于输入的振荡器所取代。通过免疫组织化学，免疫印迹和实时PCR，我们研究了一株褪黑素高和一例褪黑素低的小鼠品系在松果体中所有主要时钟基因及其产物的昼夜表达谱。在两个小鼠品系的松果体器官中也发现了所有已知的发条元素，对于SCN的持续节律产生都是必不可少的。只有mPer1 mRNA和PER1蛋白质的积累与许多其他松果体基因及其产物的时程一致，而后者是CyclampAMP诱导的。在这里，提出的数据以及在啮齿类动物松果体中调控mPer1基因的已知机制一起提出了这样的想法，即在该组织中，PER1可能具有触发功能，以启动发条的转录/翻译反馈环的循环。

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