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首页> 外文期刊>The European Journal of Neuroscience >Median raphe mediates estrogenic effects to the hippocampus in female rats.
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Median raphe mediates estrogenic effects to the hippocampus in female rats.

机译：中缝点子对雌性大鼠海马介导雌激素作用。

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Subcortical regions such as the medial septum-diagonal band of Broca and supramammillary area have been shown to mediate indirect oestrogenic effects on hippocampal morphology and function. Here, the role of the median raphe (MR), a serotonergic subcortical structure, is studied. To this end, 17beta-estradiol-filled 30-gauge cannulae were implanted into the MR of female ovariectomized rats; cholesterol-filled cannulae served as controls. After seven days, using unbiased electron microscopic stereological calculations and semiquantitative analysis, the spine synapse density and surface density of glial fibrillary acidic protein-positive astrocyte processes, respectively, were determined in the stratum radiatum of the CA1 region of the hippocampus. Changes in the serotonergic innervation of the hippocampal CA1 region were determined by immunohistochemistry and subsequent morphometric analysis. In the stratum radiatum of the CA1 region, local estradiol application into the MR resulted in a 47% increase inspine synapse density. Simultaneously, the density of glial fibrillary acidic protein-positive fibers decreased by 16%. The density of serotonin (5-HT) innervation of the strata lacunosum moleculare and radiatum of the CA1 region of the hippocampus was reduced in response to estradiol, as shown by a decrease in the length of fibers (27.6 and 48.3% decrease, respectively) and the number of large varicosities (32.5 and 38.8% decrease, respectively). These observations suggest a major role of the MR in mediating oestrogenic effects on the hippocampus and an involvement of the serotonergic system.
机译：皮质下区域，如Broca的中隔中隔对角带和上乳头区域，已显示出介导雌激素对海马形态和功能的间接作用。在这里，研究了正中缝（MR）（一种血清素能皮层下结构）的作用。为此，将17β-雌二醇填充的30号插管植入雌性去卵巢大鼠的MR中。胆固醇填充的套管用作对照。 7天后，使用无偏电子显微镜立体计算和半定量分析，分别在海马CA1区的放射状层中确定了胶质纤维酸性蛋白阳性星形胶质细胞突触的突触密度和表面密度。通过免疫组织化学和随后的形态分析来确定海马CA1区的血清素能神经支配的变化。在CA1区域的放射状层中，局部雌二醇应用到MR中导致脊柱突触密度增加47％。同时，神经胶质原纤维酸性蛋白阳性纤维的密度降低了16％。响应于雌二醇，空腹层的5-羟色胺（5-HT）神经支配密度和海马CA1区的半径减小，这是由纤维长度的减少所表示的（分别减少了27.6和48.3％）和大静脉曲张的数量（分别减少32.5和38.8％）。这些观察结果表明，MR在介导对海马的雌激素作用中发挥了主要作用，并参与了5-羟色胺能系统。

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