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首页> 外文期刊>The European Journal of Neuroscience >Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury.
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Chondroitinase ABC combined with neural stem/progenitor cell transplantation enhances graft cell migration and outgrowth of growth-associated protein-43-positive fibers after rat spinal cord injury.

机译：软骨素酶ABC结合神经干/祖细胞移植可增强大鼠脊髓损伤后移植细胞的迁移和生长相关蛋白43阳性纤维的生长。

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We previously reported that the transplantation of neural stem/progenitor cells (NSPCs) can contribute to the repair of injured spinal cord in adult rats and monkeys. In some cases, however, most of the transplanted cells adhered to the cavity wall and failed to migrate and integrate into the host spinal cord. In this study we focused on chondroitin sulfate proteoglycan (CSPG), a known constituent of glial scars that is strongly expressed after spinal cord injury (SCI), as a putative inhibitor of NSPC migration in vivo. We hypothesized that the digestion of CSPG by chondroitinase ABC (C-ABC) might promote the migration of transplanted cells and neurite outgrowth after SCI. An in vitro study revealed that the migration of NSPC-derived cells was inhibited by CSPG and that this inhibitory effect was attenuated by C-ABC pre-treatment. Consistently, an in vivo study of C-ABC treatment combined with NSPC transplantation into injured spinal cord revealed that C-ABC pre-treatment promoted the migration of the transplanted cells, whereas CSPG-immunopositive scar tissue around the lesion cavity prevented their migration into the host spinal cord in the absence of C-ABC pre-treatment. Furthermore, this combined treatment significantly induced the outgrowth of a greater number of growth-associated protein-43-positive fibers at the lesion epicentre, compared with NSPC transplantation alone. These findings suggested that the application of C-ABC enhanced the benefits of NSPC transplantation for SCI by reducing the inhibitory effects of the glial scar, indicating that this combined treatment may be a promising strategy for the regeneration of injured spinal cord.
机译：我们以前曾报道过，神经干/祖细胞（NSPC）的移植可有助于成年大鼠和猴子的脊髓损伤修复。但是，在某些情况下，大多数移植的细胞粘附在腔壁上，无法迁移并整合到宿主脊髓中。在这项研究中，我们集中研究了硫酸软骨素蛋白聚糖（CSPG），它是神经胶质疤痕的已知成分，在脊髓损伤（SCI）后被强烈表达，作为公认的NSPC体内抑制剂。我们假设软骨素酶ABC（C-ABC）消化CSPG可能会促进SCI后移植细胞的迁移和神经突的长出。一项体外研究显示，CSPG抑制了NSPC衍生细胞的迁移，而C-ABC预处理减弱了这种抑制作用。一致地，对C-ABC治疗结合NSPC移植到受损脊髓中的体内研究表明，C-ABC预处理促进了移植细胞的迁移，而病变腔周围的CSPG免疫阳性疤痕组织阻止了它们迁移到脊髓中。没有C-ABC预处理的宿主脊髓。此外，与单独进行NSPC移植相比，这种联合治疗显着诱导了病变中心的大量与生长相关的蛋白43阳性纤维的生长。这些发现表明，C-ABC的应用通过减少神经胶质瘢痕的抑制作用而增强了NSPC移植对SCI的益处，表明这种联合治疗可能是有希望的脊髓损伤再生策略。

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