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首页> 外文期刊>The European Journal of Neuroscience >Site-specific and developmental expression of pannexin1 in the mouse nervous system.
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Site-specific and developmental expression of pannexin1 in the mouse nervous system.

机译：pannexin1在小鼠神经系统中的位点特异性和发育性表达。

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摘要

Until recently, members of the connexin gene family were believed to comprise the sole molecular component forming gap junction channels in vertebrates. The recent discovery of the pannexin gene family has challenged this view, as these genes may encode for a putative second class of gap junction proteins in vertebrates. The expression of pannexin genes overlaps with those cellular networks known to exhibit a high degree of gap junctional coupling. We investigated the spatio-temporal mRNA distribution of one member of this gene family, pannexin1 (Panx1), in the brain and retina of mice using quantitative real-time polymerase chain reaction and a combination of in situ hybridization and immunohistochemistry for cellular resolution. Our results demonstrate a widespread expression of Panx1 in the brain, retina and other non-neuronal tissues. In the cortex, cerebellum and eye, Panx1 is expressed at early embryonic time points and peaks around embryonic day 18 followed by a decline towards adulthood. Most notably, Panx1 is detectable in neurons of many brain nuclei, which are known to be coupled by gap junctions as well as in previously unrecognized areas. Abundant expression was found in the adult hippocampal and neocortical pyramidal cells and interneurons, neurons of the reticular thalamus, the inferior olive, magnocellular hypothalamic neurons, midbrain and brain stem motoneurons, Purkinje cells and the retina.
机译：直到最近，人们仍认为连接蛋白基因家族的成员包括在脊椎动物中形成间隙连接通道的唯一分子组分。 Pannexin基因家族的最新发现挑战了这一观点，因为这些基因可能编码脊椎动物中第二类假定的间隙连接蛋白。泛新蛋白基因的表达与已知表现出高度间隙连接偶联的那些细胞网络重叠。我们使用定量实时聚合酶链反应以及结合原位杂交和免疫组化的细胞分辨率研究了该基因家族的一个成员pannexin1（Panx1）在小鼠的大脑和视网膜中的时空mRNA分布。我们的结果表明Panx1在脑，视网膜和其他非神经元组织中广泛表达。在皮质，小脑和眼睛中，Panx1在早期胚胎时间点表达，并在胚胎第18天左右达到峰值，然后下降到成年。最值得注意的是，Panx1在许多大脑核的神经元中是可检测到的，已知这些神经元是通过缝隙连接以及先前无法识别的区域耦合的。在成年海马和新皮质锥体细胞和中间神经元，网状丘脑神经元，下橄榄，下丘脑神经元，中脑和脑干运动神经元，浦肯野细胞和视网膜中发现大量表达。

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