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首页> 外文期刊>The European Journal of Neuroscience >Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia.
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Exposure of lentiviral vectors to subneutral pH shifts the tropism from Purkinje cell to Bergmann glia.

机译：慢病毒载体暴露于亚中性pH会使嗜性从Purkinje细胞转移至Bergmann胶质细胞。

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Cerebellar Purkinje cells play an important role in cerebellar function; lesions of Purkinje cells result in the disruption of motor coordination and motor learning. Although selective gene delivery to Purkinje cells would be a powerful technique for the study of pathophysiology in the cerebellum, a method for such a delivery has not yet been established. Here we employed human immunodeficiency virus-derived lentiviral vectors pseudotyped with vesicular stomatitis virus glycoprotein to transduce Purkinje cells and examined factors that critically affect the viral tropism for Purkinje cells. Viral vectors encoding GFP were generated using different protocols, and were then injected into the mouse cerebellum. At 7 days and 2 months post-transduction, the relative proportions of transduced Purkinje cells were determined. Lentiviral vectors harvested from a medium of pH 7.2 preferentially transduced Purkinje cells (about half of the transduced cells). In contrast, when the viral vector was harvested from medium of
机译：小脑浦肯野细胞在小脑功能中起重要作用。浦肯野细胞的损伤导致运动协调和运动学习的破坏。尽管选择性基因递送至浦肯野细胞将是研究小脑病理生理学的有力技术，但尚未建立这种递送的方法。在这里，我们采用水泡性口炎病毒糖蛋白假型化的人免疫缺陷病毒衍生的慢病毒载体转导浦肯野细胞，并研究了严重影响浦肯野细胞病毒嗜性的因素。使用不同的协议生成编码GFP的病毒载体，然后将其注射到小鼠小脑中。转导后7天和2个月，测定转导的浦肯野细胞的相对比例。从pH 7.2的培养基中收获的慢病毒载体优先转导了浦肯野细胞（大约一半的转导细胞）。相反，当从pH≤7.0的培养基中收获病毒载体时，只有12-26％的被转导细胞被鉴定为浦肯野细胞，而68-77％的被鉴定为伯格曼胶质细胞。在解离的细胞培养物中，观察到浦肯野细胞转导效率的类似降低，这取决于病毒收获时培养基的pH。这些结果表明，浦肯野细胞的慢载体趋向性对pH极为敏感：收获时培养基pH的细微降低使病毒的向性向Bergmann胶质细胞转移。

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