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首页> 外文期刊>The European Journal of Neuroscience >ATF3 expression in L4 dorsal root ganglion neurons after L5 spinal nerve transection.
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ATF3 expression in L4 dorsal root ganglion neurons after L5 spinal nerve transection.

机译：L5脊神经横断后L4背根神经节神经元中ATF3的表达。

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摘要

Activating transcription factor 3 (ATF3) is a widely used marker of damaged primary sensory neurons that is induced in essentially all dorsal root ganglion (DRG) neurons by spinal nerve axotomy. Whether such injuries induce its expression in neurons of adjacent DRGs remains unknown. Following L5 spinal nerve ligation, experimental but not sham-operated rats develop thermal and mechanical hypersensitivity. In the L4 DRG, 11-12% of neurons were ATF3 positive by 1 day post-surgery, and numbers remain unchanged at 2 weeks. Importantly, sham exposure of the L5 spinal nerve produced a nearly identical number of ATF3-positive neurons in the L4 DRG and also a substantial increase in the L5 DRG, with a similar time-course to experimental animals. There was no correlation between behaviour and magnitude of ATF3 expression. Co-localization studies with the DRG injury markers galanin, neuropeptide Y and nitric oxide synthase (NOS) showed that approximately 75, 50 and 25%, respectively, of L4 ATF3-positive neurons co-expressed these markers after L5 transection or sham surgery. Additionally, increases in galanin and NOS were seen in ATF3-negative neurons in L4. Our results strongly suggest that the surgical exposure of spinal nerves induces ATF3 in the L4-5 DRG, irrespective of whether the L5 nerve is subsequently cut. This probably reflects minor damage to the neurons or their axons but nevertheless is sufficient to induce phenotypic plasticity. Caution is therefore warranted when interpreting the phenotypic plasticity of DRG neurons in adjacent ganglia in the absence of positive evidence that they are not damaged.
机译：激活转录因子3（ATF3）是受损的初级感觉神经元的一种广泛使用的标志物，基本上由脊髓神经轴突切开术在所有背根神经节（DRG）神经元中诱导。这种损伤是否在邻近DRG的神经元中诱导其表达尚不清楚。 L5脊髓神经结扎后，实验性但非假手术的大鼠发生热和机械超敏反应。在L4 DRG中，术后1天神经元的ATF3阳性率为11-12％，并且在2周时数量保持不变。重要的是，假暴露L5脊髓神经在L4 DRG中产生了几乎相同数量的ATF3阳性神经元，并且在L5 DRG中也产生了实质性的增加，其时间过程与实验动物相似。在行为和ATF3表达的大小之间没有相关性。与DRG损伤标记甘丙肽，神经肽Y和一氧化氮合酶（NOS）的共定位研究表明，在L5横切或假手术后，分别约有75、50和25％的L4 ATF3阳性神经元共同表达了这些标记。另外，在L4的ATF3阴性神经元中可见甘丙肽和NOS增加。我们的结果强烈表明，无论随后是否切除L5神经，手术暴露于脊神经都会在L4-5 DRG中诱导ATF3。这可能反映了对神经元或其轴突的轻微损害，但仍足以诱导表型可塑性。因此，在缺乏周围神经节DRG神经元未受损的积极证据的情况下，在解释DRG神经元的表型可塑性时应格外小心。

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