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首页> 外文期刊>The European Journal of Neuroscience >Populations of hippocampal inhibitory neurons express different levels of cytochrome c.
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Populations of hippocampal inhibitory neurons express different levels of cytochrome c.

机译：海马抑制性神经元群体表达不同水平的细胞色素c。

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Cytochrome c (CC) immunoreactivity was quantified in functionally distinct rat hippocampal inhibitory neuron populations using double immunocytochemistry and laser scanning confocal microscopy to measure the CC expression level as well as the amount of mitochondria within the cells, which is a sign of neuronal activity. The CC signal showed a similar distribution to cytochrome c oxidase histochemical staining. Strongly stained somata, dendrites and axon terminal clouds were dispersed over the low intensity neuropil staining. The staining was granular and electron microscopic investigation confirmed that the signal was localized in mitochondria. Intensively labeled neurons, showing the morphological features of inhibitory cells, were most frequently found in the principal cell layers, stratum oriens of the CA1-3 areas, stratum lucidum and hilus. These neurons contained not only a higher number of mitochondria than the principal cells but the intensity of the mitochondrial staining was evidently stronger. Among the examined interneuronal populations, parvalbumin-immunoreactive neurons were intensively labeled for CC. Calbindin D28k- (CB), somatostatin- and cholecystokinin-labeled cells showed heterogeneous CC levels, whereas calretinin-immunoreactive cells never showed a strong CC signal. CB cells in stratum oriens and alveus layers, lucidum and the hilus were strongly labeled for CC. CB cells in such regions are known to project to the medial septum and contain somatostatin. We have demonstrated that the CA1 interneurons that project to the medial septum (hippocampo-septal neurons) express a high level of CC. Thus, similar to the parvalbumin-containing basket and axo-axonic cells, the hippocampo-septal neurons potentially have a high average activity level.
机译：使用双重免疫细胞化学和激光扫描共聚焦显微镜对功能不同的大鼠海马抑制性神经元群体中的细胞色素c（CC）免疫反应进行定量，以测量CC表达水平以及细胞内线粒体的量，这是神经元活性的标志。 CC信号显示出与细胞色素C氧化酶组织化学染色相似的分布。强烈染色的躯体，树突和轴突终末云散布在低强度神经纤维染色上。染色呈颗粒状，电子显微镜检查证实该信号位于线粒体中。在主要的细胞层，CA1-3区域的原始层，透明质层和hilus中，发现频率最高的显示抑制性细胞形态特征的密集标记神经元。这些神经元不仅比主要细胞包含更多的线粒体，而且线粒体染色的强度明显更强。在所检查的神经元间群体中，小白蛋白免疫反应性神经元被强烈标记为CC。钙结合蛋白D28k-（CB），生长抑素和胆囊收缩素标记的细胞显示出不同的CC水平，而钙卫蛋白免疫反应性细胞则从未显示出强CC信号。 Oriens和肺泡层，透明质和hilus中的CB细胞被强烈标记为CC。已知在此类区域中的CB细胞可投射到中隔，并含有生长抑素。我们已经证明，投射到内侧中隔（海马间隔神经元）的CA1神经元表达高水平的CC。因此，类似于含有小白蛋白的篮子和轴突细胞，海马-中隔神经元可能具有较高的平均活性水平。

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