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首页> 外文期刊>The European Journal of Neuroscience >Microarray-based long-term detection of genes differentially expressed after cortical spreading depression.
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Microarray-based long-term detection of genes differentially expressed after cortical spreading depression.

机译：基于微阵列的基因在皮质扩散抑制后差异表达基因的长期检测。

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摘要

Spreading depression (SD) is a slowly propagating wave of neuronal depolarization altering ion homeostasis, blood flow and energy metabolism without causing irreversible damage of the tissue. As SD has been implicated in several neurological diseases including migraine and stroke, understanding these disorders requires systematic knowledge of the processes modified by SD. Thus, we induced repetitive SD in the rat cerebral cortex by topical application of 3 m KCl for approximately 2 h and evaluated the kinetics of SD-induced changes in cortical gene expression for up to 30 days using Affymetrix RAE230A arrays. The temporal profile showed a rapid expression of immediate early genes, genes associated with inflammation, metabolism, stress and DNA repair, ion transport, and genes that play a role in growth/differentiation. Stress-response genes could still be detected after 24 h. At this time, induced genes were mainly related to the cell membrane and adhesion, or to the cytoskeleton. A subset of genes was still affected even 30 days after SD. Real-time polymerase chain reactions and immunohistochemistry confirmed the microarray results for several of the transcripts. Our findings demonstrate a temporal pattern of gene expression which might promote tissue remodeling and cortical plasticity, and might probably account for the mediation of neuronal tolerance towards subsequent ischemia.
机译：散发性抑郁症（SD）是神经元去极化的缓慢传播波，可改变离子稳态，血流和能量代谢，而不会引起组织的不可逆损伤。由于SD已牵涉到包括偏头痛和中风在内的多种神经系统疾病，因此了解这些疾病需要系统地了解SD修饰的过程。因此，我们通过局部应用3 m KCl约2 h诱导大鼠大脑皮层中的重复性SD，并使用Affymetrix RAE230A阵列评估了SD诱导的皮质基因表达变化长达30天的动力学。时间分布图显示了快速早期基因，与炎症，代谢，应激和DNA修复，离子转运相关的基因以及在生长/分化中起作用的基因的快速表达。 24小时后仍可检测到应激反应基因。这时，诱导的基因主要与细胞膜和粘附或细胞骨架有关。甚至在SD后30天，仍有一部分基因受到影响。实时聚合酶链反应和免疫组织化学证实了一些转录本的微阵列结果。我们的发现表明基因表达的时间模式可能促进组织重构和皮质可塑性，并可能解释了对后续缺血的神经元耐受的调节。

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